Medicinal properties of plants is been studied since long back and various research on it was carried out and from that various pharmaceutical, health care, cosmetic products are been manufactured. This research was aim to formulate the herbal sanitizer. Sanitizer formulated from neem and lemon juice. Phytochemical and antioxidant properties of neem extract and lemon juice were analysed. Their MIC was checked against standard culture and hand isolates from lab assistance. MIC of neem extract and lemon juice was found. Three different sanitizers were formulated lemon, lemon-neem and neem sanitizer. Formulated sanitizer was compared with sterillium sanitizer and it was found that sterillium was effective against standard organism only and not against hand isolates while formulated sanitizer was effective against both. Even in comparison with respect to zone of inhibition, formulated sanitizers were showing more zones as compared to sterillium sanitizer. Antibacterial, antifungal and antibiofilm activity of formulated herbal sanitizer was studied. Antibiotic potentiation activity of formulated sanitizer was tested against Methicillin Resistant Staphylococcus aureus and isolate IV to find out change in the sensitivity pattern of antibiotics. Finally lemon, lemon-neem and neem sanitizer efficacy was tested. Result obtained showed reduction in bacterial growth after treatment with sanitizer. As neem sanitizer was tested found to be bitter hence instead of using it as hand sanitizer it can be use as a spray on medical devices or as a floor cleaner.

Keywords : Sanitizer, MIC, Antibacterial.