Herbs are obtained from natural sources. Now a days they are being incorporated into cosmeceutical formulations due to the presence of various properties like anti-bacterial, anti-viral, anti- oxidant and anti-inflammatory activities. The goal of the present study was to formulate and evaluate herbal lipstick, screening of phytochemicals and to provide in- vitro evidence for radical scavenging activity of Ethanolic flower extract of Brassica Oleraceae var. Italica, characterization of bioactive compound by chromatographic techniques and its quantification using analytical technique. Phyto-chemical screening of extract was performed which indicated the presence of many active constituents. Lipstick was formulated into five different formulations and evaluation tests were performed. The evaluation test results shown that fifth formulation has good stability than others when compared to marketed product. The broccoli extract had shown good antioxidant activity when compared with standard ascorbic acid. The total phenolic content of broccoli extract was determined and expressed in gallic acid equivalents (36.2 ± 0.9). TLC was performed using quercetin as standard. The bioactive component was detected as quercetin based on the proximity of Rf value of sample extract and Rf value of standard quercetin. A simple, rapid and sensitive method was developed for HPTLC quantitative analysis and results shown that 100mg of broccoli extract contains 29.6mg of quercetin. The herbal lipstick BLISS, formulated by us using broccoli flower extract containing quercetin, a flavanoid which being an antioxidant, reduces oxidative stress and prevents photoaging of lips keeping it supple and luscious in the long run.

Keywords : Broccoli, Lipstick, Photoageing, Antioxidant activity, Quercetin, HPTLC Analysis.