Over the past decade, there has often been a shortage of Zn and Fe simultaneously. In cell culture, Zn and Fe are micronutrient substances that can increase immune responses so that the number of malaria parasite cultures is decreased or negative. The presence of Zn and Fe as micronutrients in the body is influenced by nutritional status. A child can be considered to have a good nutritional status if the micronutrient elements in the body are fulfilled. This study aims to analyze the expression of IL10 and IL-12 children suffering malaria by providing Zinc and FE supplementation to elementary school children in malaria-endemic area. This experimental study gave treatments to the research subjects, divided into four groups, including the treatment group with @ 20 mg/day Zinc (Zn), Fe @ 20 mg/day, Zn and Fe @ 20 mg/day simultaneously and the control group (placebo). The dose given in this study was 20 mg. This study employed the Randomized Controlled Trial Pre-Post Test Design by providing a Double-Blind treatment. Further, the data were statistically analyzed using one way ANOVA analysis with 5% reliance level ( = 0.05). The results show that the level of IL-12 is increased after the treatment of Fe and Zn supplementation; meanwhile, the level of IL-10 is decreased. The approach that can describe this situation is the presence of seasonal malaria, low transmissibility, low percentage of API and the Zn levels which are up to normal in the 9 to 12-year-old children. This causes the increased protective IL-12 level so that the IL-10 level is no longer needed to re-stabilize the level of IL-12 so that the level of IL-10 in this study is decreased.

Keywords : Zn and Fe, IL-10, IL-12, Malaria.