Authors :
Saffia Shaheen; Zertashia Akram; Tehmina Mezhar; Ishrat Mahjabeen; Muhammad Rizwan
Volume/Issue :
Volume 6 - 2021, Issue 12 - December
Google Scholar :
http://bitly.ws/gu88
Scribd :
https://bit.ly/3eeTSR7
Abstract :
Poly (ADP-Ribose) Polymerase1, PARP1 is an
important protein coding gene that involve in DNA
repair mechanism. It acts as a first responder that
detects DNA damage and then facilitates choice for
repair pathways. The current study was design to study
the expression variation of PARP1 gene in salt mine
workers. In the present study 200 blood samples of
exposed salt mine workers along with 200 control groups
were used. Expression variety of PARP1 quality was
assessed by qPCR. Relative expression of PARP1 was
(p<0.01) up regulated in exposed groups compared with
control groups. Relative expression of PARP1 gene
increased significantly in worker group with >30-year
age compared to their respective controls (P<0.01) and
even to worker group with <30-year age (P<0.05). The
expression of PARP1 gene seen unregulated in mine who
work in mine more than 10 years. Study cohort of
workers and control group was divided as smokers and
non-smokers. Significant (P<0.05) up regulation in
PARP1 expression was seen in smoker category of mine
workers compared with control smokers and nonsmokers of mine workers. Mine workers divided into
groups based on type of fuel used by workers performing
duty as workers using gas or wood. Significant (P<0.05)
up regulation of PARP1 gene was observed in mine
group working with wood compared to group working
with gas. The obtained results suggest that salt mine
workers are exposed to different genotoxins at their
workplaces which affect the genetic integrity and DNA
repair capacity.
Poly (ADP-Ribose) Polymerase1, PARP1 is an
important protein coding gene that involve in DNA
repair mechanism. It acts as a first responder that
detects DNA damage and then facilitates choice for
repair pathways. The current study was design to study
the expression variation of PARP1 gene in salt mine
workers. In the present study 200 blood samples of
exposed salt mine workers along with 200 control groups
were used. Expression variety of PARP1 quality was
assessed by qPCR. Relative expression of PARP1 was
(p<0.01) up regulated in exposed groups compared with
control groups. Relative expression of PARP1 gene
increased significantly in worker group with >30-year
age compared to their respective controls (P<0.01) and
even to worker group with <30-year age (P<0.05). The
expression of PARP1 gene seen unregulated in mine who
work in mine more than 10 years. Study cohort of
workers and control group was divided as smokers and
non-smokers. Significant (P<0.05) up regulation in
PARP1 expression was seen in smoker category of mine
workers compared with control smokers and nonsmokers of mine workers. Mine workers divided into
groups based on type of fuel used by workers performing
duty as workers using gas or wood. Significant (P<0.05)
up regulation of PARP1 gene was observed in mine
group working with wood compared to group working
with gas. The obtained results suggest that salt mine
workers are exposed to different genotoxins at their
workplaces which affect the genetic integrity and DNA
repair capacity.
