Evaluation of the Immunodot Technique Compared to the Elisa Technique for the Detection of IgA Antibodies to Tissue Transglutaminase


Authors : Oussama Aazzane; Hasnaa Bazhar; Hassan Fellah

Volume/Issue : Volume 9 - 2024, Issue 12 - December

Google Scholar : https://tinyurl.com/3spt22xf

Scribd : https://tinyurl.com/4422wkw9

DOI : https://doi.org/10.5281/zenodo.14576664

Abstract : Celiac disease (CD) is a chronic autoimmune enteropathy characterized by intestinal villous atrophy secondary to gluten ingestion. In the biological diagnosis of CD, serology includes, among other tests, the search for IgA anti-tissue transglutaminase antibodies (IgA anti-tTG). Various techniques are employed, including ELISA and Immunodot. The objective of this work is to evaluate the diagnostic performance of the Immunodot test compared to the ELISA test for detecting IgA anti- tTG antibodies in the serum of patients with CD and controls. This is a comparative study of the Immunodot test against the ELISA test for the detection of IgA anti- tTG antibodies. The antibody dosage was performed on 96 samples belonging to 49 patients with CD and 47 healthy subjects. All samples were tested using the Immunodot test (DotDiver CeliAK IgA) and the ELISA test (Anti-tissue Transglutaminase Elisa (IgA) Test instruction). The comparison of the Immunodot test to the ELISA test revealed a sensitivity of 59% and a negative predictive value (NPV) of 73%.

Keywords : Celiac Disease, Anti-TG Antibodies, Immunodot, Elisa, IgA.

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Celiac disease (CD) is a chronic autoimmune enteropathy characterized by intestinal villous atrophy secondary to gluten ingestion. In the biological diagnosis of CD, serology includes, among other tests, the search for IgA anti-tissue transglutaminase antibodies (IgA anti-tTG). Various techniques are employed, including ELISA and Immunodot. The objective of this work is to evaluate the diagnostic performance of the Immunodot test compared to the ELISA test for detecting IgA anti- tTG antibodies in the serum of patients with CD and controls. This is a comparative study of the Immunodot test against the ELISA test for the detection of IgA anti- tTG antibodies. The antibody dosage was performed on 96 samples belonging to 49 patients with CD and 47 healthy subjects. All samples were tested using the Immunodot test (DotDiver CeliAK IgA) and the ELISA test (Anti-tissue Transglutaminase Elisa (IgA) Test instruction). The comparison of the Immunodot test to the ELISA test revealed a sensitivity of 59% and a negative predictive value (NPV) of 73%.

Keywords : Celiac Disease, Anti-TG Antibodies, Immunodot, Elisa, IgA.

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