Authors :
Oussama Aazzane; Hasnaa Bazhar; Hassan Fellah
Volume/Issue :
Volume 9 - 2024, Issue 12 - December
Google Scholar :
https://tinyurl.com/3spt22xf
Scribd :
https://tinyurl.com/4422wkw9
DOI :
https://doi.org/10.5281/zenodo.14576664
Abstract :
Celiac disease (CD) is a chronic autoimmune
enteropathy characterized by intestinal villous atrophy
secondary to gluten ingestion. In the biological diagnosis
of CD, serology includes, among other tests, the search
for IgA anti-tissue transglutaminase antibodies (IgA
anti-tTG). Various techniques are employed, including
ELISA and Immunodot. The objective of this work is to
evaluate the diagnostic performance of the Immunodot
test compared to the ELISA test for detecting IgA anti-
tTG antibodies in the serum of patients with CD and
controls. This is a comparative study of the Immunodot
test against the ELISA test for the detection of IgA anti-
tTG antibodies. The antibody dosage was performed on
96 samples belonging to 49 patients with CD and 47
healthy subjects. All samples were tested using the
Immunodot test (DotDiver CeliAK IgA) and the ELISA
test (Anti-tissue Transglutaminase Elisa (IgA) Test
instruction). The comparison of the Immunodot test to
the ELISA test revealed a sensitivity of 59% and a
negative predictive value (NPV) of 73%.
Keywords :
Celiac Disease, Anti-TG Antibodies, Immunodot, Elisa, IgA.
References :
- Tasuku T,Kenji I. Celiac Disease Genetics, Pathogenesis, and Standard Therapy for Japanese Patients. International Journal of Molecular Sciences. 2023 Jan 20 ;24(3) :2075. doi: 10.3390/ijms24032075.
- KHATER S, CELLIER C. Celiac disease: diagnosis and treatment. Journal of Medical Biology. 2020.
- National Institutes of Health consensus development conference statement on celiac disease, June 28–30, 2004. Gastroenterology. 2005; 128(4 Suppl 1):S1–9. doi: 10.1053/j.gastro.2005.02.007.
- Fasano A, Catassi C. Clinical practice. Celiac disease. The New England Journal of Medicine. 2012 ; 367(25) :2419–26. doi.org/10.1056/NEJMcp1113994.
- Kelly CP, Bai JC, Liu E, Leffler DA. Advances in diagnosis and management of celiac disease. Gastroenterology. 2015; 148(6):1175–86. doi: 10.1053/j.gastro.2015.01.044.
- Malamut G, Cellier C. Celiac disease. EMC. 2016. doi: 10.1016/S1634-6939(16)60111-4.
- Husby S, Koletzko S, Korponay-Szabó I, et al. European Society Paediatric Gastroenterology, Hepatology and Nutrition Guidelines for Diagnosing Coeliac Disease 2020. Journal of Pediatric Gastroenterology and Nutrition. 2020 Jan;70(1):141-156. doi: 10.1097/MPG.0000000000002497.
- Khadiry B, Fellah H, et al. Evaluation of the ELISA technique compared to Immunodot in the screening of extractable nuclear antigens antibodies Sm, SSA and SSB. International Journal of Innovation and Applied Studies. 2022, May, pp. 493-501.
- Ousmane S, El Fakir S, El Rhazi K, et al. Estimating the roc curve. Moroccan Journal of Public Health. 2015, vol 2, n° 2.
- Schaefer L, Dyke J , Meglio F, et al. Evaluation of microparticle enzyme immunoassays for immunoglobulins G and M to rubella virus and Toxoplasma gondii on the Abbott IMx automated analyzer. Journal of Clinical Microbiology.1989 Nov; 27(11):2410-3. doi: 10.1128/jcm.27.11.2410-2413.1989.
Celiac disease (CD) is a chronic autoimmune
enteropathy characterized by intestinal villous atrophy
secondary to gluten ingestion. In the biological diagnosis
of CD, serology includes, among other tests, the search
for IgA anti-tissue transglutaminase antibodies (IgA
anti-tTG). Various techniques are employed, including
ELISA and Immunodot. The objective of this work is to
evaluate the diagnostic performance of the Immunodot
test compared to the ELISA test for detecting IgA anti-
tTG antibodies in the serum of patients with CD and
controls. This is a comparative study of the Immunodot
test against the ELISA test for the detection of IgA anti-
tTG antibodies. The antibody dosage was performed on
96 samples belonging to 49 patients with CD and 47
healthy subjects. All samples were tested using the
Immunodot test (DotDiver CeliAK IgA) and the ELISA
test (Anti-tissue Transglutaminase Elisa (IgA) Test
instruction). The comparison of the Immunodot test to
the ELISA test revealed a sensitivity of 59% and a
negative predictive value (NPV) of 73%.
Keywords :
Celiac Disease, Anti-TG Antibodies, Immunodot, Elisa, IgA.