Authors :
Patani Inemotimineri; Ogundu, Uduak Emmanuel; Okoro, Victor Mela Obinna; Kadurumba, Ogechi Evangeline
Volume/Issue :
Volume 8 - 2023, Issue 12 - December
Google Scholar :
http://tinyurl.com/hhhk85cz
Scribd :
http://tinyurl.com/3srrh69e
DOI :
https://doi.org/10.5281/zenodo.10473256
Abstract :
This study evaluated the type of mutation
associated with SNPs of black skinned and white skinned
snails (Archachatina marginata) in the rainforest zone of
Nigeria. Fourty eight (48) Archachatina marginata snails
were assigned to two treatments using completely
randomized design. The two treatments consisted of
twenty four (24) white skinned ecotypes and twenty
four(24) black skinned ecotypes with 12 replicates each.
These snails were taken to laboratory to conduct DNA
analysis to evaluate the mutation type that is associated
with the SNPs of the two snail ectotypes. The quality of
DNA was assessed using gel electrophoresis. Data collected
was subjected to statistical analysis using Mega 7.0,
DNAsp version 5 and GENPOP software packages.
Mutation result obtained in this study revealed that both
types undergo both substitution and deletion with deletion
being predominant in the black and substitution in the
white skinned A. marginata. It was also observed that the
codons mostly affected by deletion are the nucleotides
coding for amino acids phenyl alanine and alanine. These
amino acids are responsible for growth, immunity and skin
colouration. Since the immune-related genes are absent in
black people, the high rate of deletion that black
experience may be the cause of their high mortality rate.
Self-fertilization, a high degree of inbreeding, and
restricted gene flow all contribute to the high rate of
deletion. Thus, it would be beneficial to promote the
crossbreeding of A. marginata snails with black and white
skin.
Keywords :
Mutation, Evaluation, Substitution, Deletion.
This study evaluated the type of mutation
associated with SNPs of black skinned and white skinned
snails (Archachatina marginata) in the rainforest zone of
Nigeria. Fourty eight (48) Archachatina marginata snails
were assigned to two treatments using completely
randomized design. The two treatments consisted of
twenty four (24) white skinned ecotypes and twenty
four(24) black skinned ecotypes with 12 replicates each.
These snails were taken to laboratory to conduct DNA
analysis to evaluate the mutation type that is associated
with the SNPs of the two snail ectotypes. The quality of
DNA was assessed using gel electrophoresis. Data collected
was subjected to statistical analysis using Mega 7.0,
DNAsp version 5 and GENPOP software packages.
Mutation result obtained in this study revealed that both
types undergo both substitution and deletion with deletion
being predominant in the black and substitution in the
white skinned A. marginata. It was also observed that the
codons mostly affected by deletion are the nucleotides
coding for amino acids phenyl alanine and alanine. These
amino acids are responsible for growth, immunity and skin
colouration. Since the immune-related genes are absent in
black people, the high rate of deletion that black
experience may be the cause of their high mortality rate.
Self-fertilization, a high degree of inbreeding, and
restricted gene flow all contribute to the high rate of
deletion. Thus, it would be beneficial to promote the
crossbreeding of A. marginata snails with black and white
skin.
Keywords :
Mutation, Evaluation, Substitution, Deletion.
