Authors :
Onuoha, U.N.; Appeh, G.O.
Volume/Issue :
Volume 8 - 2023, Issue 6 - June
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/4r99wmut
DOI :
https://doi.org/10.5281/zenodo.8149736
Abstract :
The beneficial effects of bioremediation with
oil-degrading microorganism, Pseudomonas aeruginosa
and Bacillus subtilis, on tomato seedlings growth in oil-
contaminated soil was investigated. The effect of
bioremediation was examined with Pseudomonas
aeruginosa and Bacillus subtilis on phytotoxicity, as
measured by tomato seed growth. Tomato growth
parameters (leaf length, height, seed germination,
chlorosis, stunted growth) were evaluated daily for
15days.Some qualitative morphological
observations made on the seedlings were also observed
for seedlings. In the untreated control as well as the
treated experimental samples (Pseudomonas aeruginosa
and Bacillus subtilis), sprouting of the seeds were rapid
and completed in a few days, the seedlings showed steady
growth and morphologically normal. In the control soils
however, sprouting was sluggish, and growth was
stunted, yellowing of the leaves was observed (chlorosis);
a number of the seedlings were folded at the tips. These
were indications of phytotoxicity resulting from oil
pollution. The results of the present research have
proven the bioremediation potential of bacterial isolates,
for crude oil contamination which also showed
significant growth potential for the tomato seeds.
Keywords :
Phytotoxicity, Pollution, Crude Oil, Pseudomonas aeruginosa, Tomatoes.
The beneficial effects of bioremediation with
oil-degrading microorganism, Pseudomonas aeruginosa
and Bacillus subtilis, on tomato seedlings growth in oil-
contaminated soil was investigated. The effect of
bioremediation was examined with Pseudomonas
aeruginosa and Bacillus subtilis on phytotoxicity, as
measured by tomato seed growth. Tomato growth
parameters (leaf length, height, seed germination,
chlorosis, stunted growth) were evaluated daily for
15days.Some qualitative morphological
observations made on the seedlings were also observed
for seedlings. In the untreated control as well as the
treated experimental samples (Pseudomonas aeruginosa
and Bacillus subtilis), sprouting of the seeds were rapid
and completed in a few days, the seedlings showed steady
growth and morphologically normal. In the control soils
however, sprouting was sluggish, and growth was
stunted, yellowing of the leaves was observed (chlorosis);
a number of the seedlings were folded at the tips. These
were indications of phytotoxicity resulting from oil
pollution. The results of the present research have
proven the bioremediation potential of bacterial isolates,
for crude oil contamination which also showed
significant growth potential for the tomato seeds.
Keywords :
Phytotoxicity, Pollution, Crude Oil, Pseudomonas aeruginosa, Tomatoes.
