Evaluating the Effectiveness of Soil-Derived Microbes in Degrading Pestricides using Nms Media and their Impact on Fenugreek Growth


Authors : Vaishnavi Rajendra Shelke

Volume/Issue : Volume 10 - 2025, Issue 3 - March


Google Scholar : https://tinyurl.com/386xepkb

Scribd : https://tinyurl.com/4swp248b

DOI : https://doi.org/10.38124/ijisrt/25mar392

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : Pesticide contamination in agricultural soil has become a major environmental concern, leading to soil degradation and adversely affecting plant growth. This study focuses on isolating microbial strains from pesticide- contaminated soil collected from agricultural fields in Nashik, India, and evaluating their ability to degrade pesticides. Three microbial strains (Sample A, Sample B, and Sample C) were isolated using the serial dilution and spread plate method. These microbes were inoculated into Nitrate Mineral Salts (NMS) medium containing pesticides to assess their degradation efficiency through optical density (OD) measurements. And after the successful degradation, the degraded solution was applied to fenugreek plants to observe its impact on plant growth compared to a control. The results was observed that the microbial strains efficiently degraded pesticides, significantly reducing toxicity and enhancing soil quality, which led to improved plant growth. This study it is was observed that microbial bioremediation could be a sustainable approach to reducing the pesticide toxicity in agricultural soil.

Keywords : Pesticide Degradation, Microbial Bioremediation, Soil Microbes, Plant Growth, Optical Density.

References :

  1. Indratin, A., Kurnia, A., and Wahyuni, S., "Degradation of Cypermethrin by Indigenous Bacteria from Contaminated Soil," Indonesian Agricultural Environment Research Institute (IAERI), 2019.
  2. Smith, J. et al., "Microbial Degradation of Pesticides," Journal of Environmental Science, 2020.

Pesticide contamination in agricultural soil has become a major environmental concern, leading to soil degradation and adversely affecting plant growth. This study focuses on isolating microbial strains from pesticide- contaminated soil collected from agricultural fields in Nashik, India, and evaluating their ability to degrade pesticides. Three microbial strains (Sample A, Sample B, and Sample C) were isolated using the serial dilution and spread plate method. These microbes were inoculated into Nitrate Mineral Salts (NMS) medium containing pesticides to assess their degradation efficiency through optical density (OD) measurements. And after the successful degradation, the degraded solution was applied to fenugreek plants to observe its impact on plant growth compared to a control. The results was observed that the microbial strains efficiently degraded pesticides, significantly reducing toxicity and enhancing soil quality, which led to improved plant growth. This study it is was observed that microbial bioremediation could be a sustainable approach to reducing the pesticide toxicity in agricultural soil.

Keywords : Pesticide Degradation, Microbial Bioremediation, Soil Microbes, Plant Growth, Optical Density.

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