Authors :
Oluchi Rose Colette Nlemolisa; Ugochi Nneka Kemka; Vivian Kelechi Gaius-Mbalisi; Peter Enoch Odonye; Romanus Chukwudi Nwokorie; Chidimma Onyinye Ikeme; Favour Chinonso Madubugo
Volume/Issue :
Volume 11 - 2026, Issue 5 - May
Google Scholar :
https://tinyurl.com/4u8nx7ya
Scribd :
https://tinyurl.com/36m2chff
DOI :
https://doi.org/10.38124/ijisrt/26May971
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
Soaps are recognized primarily as cleaning agents due to their antibacterial properties. The antibacterial efficacy of
four distinct soaps, specifically a beauty soap, medicated soap, black soap, and local soap, was examined. Standard
microbiological procedures, were employed for the identification of the isolates. The disc diffusion assay was employed to
investigate the antibacterial efficacy of the soap samples against the isolates at various concentrations: 200 mg/ml, 100 mg/ml,
50 mg/ml, 25 mg/ml, and 12.5 mg/ml. The bacteria were identified as Escherichia coli, Staphylococcus sp., and Bacillus sp. At a
concentration of 200 mg/ml, local soap inhibited the growth of all isolates, with the maximum inhibition measuring 31 mm and
the minimum inhibition measuring 8.0 mm. At a concentration of 100 mg/ml, black soap and local soap exhibited significant
inhibition against all isolates, with the zone of inhibition decreasing as the concentration of the soap samples decreased. Beauty
soap shown antibacterial activity at concentrations of 100 mg/ml and 50 mg/ml. The minimum inhibitory concentrations (MIC)
for the isolates varied between 12.5 mg/ml and 25 mg/ml, whereas the minimum bactericidal concentrations (MBC) for black
soap and local soap were determined to be 50 mg/ml and 25 mg/ml, respectively, against the isolates. Black soap and local soap,
exhibiting superior efficacy at low concentrations, may be regarded as effective antibacterial agents for cleansing purposes. The
evaluation of antimicrobial efficacy for all four soaps indicated that black soap and Local soap had superior effectiveness against
all isolates in comparison to medicated and beauty soap.
Keywords :
Medicated, Soap, Organic, Microorganism, Skin.
References :
- Anyiam, I. V. &Obulie, M. A. (2016). Effect of medicated soaps on the bacterial isolates of skins of female Students from Madonna University, Elele, Rivers State, Nigeria. Nigerian Journal of Microbiology, 30(1):3335-3338.
- Bhat, P. R., Prajna, P. S., Menezez, V. P. & Shetty, P. (2011). Antimicrobial Activities of Soap and Detergents. Advance Bioresistance, 2:52-62.
- Chaudhary, V. M. (2016). Studies on antimicrobial activity of antiseptic soaps and herbal soaps against selected human pathogens. Journal of Science and Innovative Resistance, 5(6): 201-204.
- Desbois, A.P. & Smith, V.J. (2010). Antibacterial free fatty acids: Activities mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology, 85(6), 1629– 1642.
- Ekwenye, U. N. &Ijeomah, C.A. (2015).Antimicrobial effect of palm kernel oil used in production of local soaps. KMTL Science Journal, 6: 22-26.
- Feroze, K., Elsayed, A. & Tarek, T. A (2014). Antimicrobial activity of commercial “antibacterial” handwashes and soaps. Journal of Indian Dermatology,5(3):344-346.
- Fuls, J.L., Rodgers, N.D., Fischler, G.E., Howard, J. M., Patel, M. & Weidner, P. L.(2018).‘Alternative hand contamination technique to compare the activities of antimicrobial and non-antimicrobial soaps under different test conditions’, Journal of Applied & Environmental Microbiology, 74(12), 3739–3744.
- Geraldo, I. M., Gilman, A., Shintre, M. S. & Modak, S. M. (2018). Rapid antibacterial activity of two novel hand soaps: Evaluation of the risk of development of bacterial resistance to the antibacterial agents. Infection Control Hospital Epidemiology, 29:736-41.
- Grice, E. A. & Segre, J. A. (2011). “The skin microbiome”. Nature Reviews Microbiology, 9,244–253.
- Ikegbunam, M., Metuh, R., Anagu, L. & Nsikak, N. (2013). Antimicrobial activity of some cleaning products against selected bacteria. International Resources Journal Pharmaceutical and Applied Sciences, 3(4):133-135.
- Kingsley, T.U., Kelechi, E. E. & Chukwudi, U.A. (2019). Antibacterial activities of medicated soaps on selected clinical bacterial isolates, Journal of Basic Pharmacology Toxicology,3(2):17-20.
- Lonsway, D. (2021). Preparation of routine media and reagents used in antimicrobial susceptibility testing, Clinical Microbiology Procedures Handbook, 5th Edition. ASM Press, Washington, DC.p.5.20.1.1-5.20.1.4
- Njoku-Tony, R.F., Ebe, T.E., Ihejirika, C.E., Ejiogu, C.C. & Uyo, C.N. (2016). Assessment of Physicochemical and Microbial Load of Nworie River Owerri, Imo State, South-Eastern Nigeria, IOSR Journal of Environmental Science, Toxicology and Food Technology, 10:67- 75.
- Nnema, E. E. (2017). Evaluation of Antibacterial Effects of Dettol and Eva Soaps. Nigerian Journal of Microbiology, 31(1): 3746-3750.
- Obi, C. N. (2014). Antibacterial activities of some medicated soaps on selected human pathogens.Annual Journal of Microbiology, 2(6):178-181.
- Oladosu, P. O., Umar, Y. A., Salawudeen, A., Izebe, K., Adamu, M. T. & Aboh, M. (2018). Antibacterial activity of soaps indigenously made in Gombe Metropolis, Nigeria. Journal of Natural Remedies, 18(4):123-130.
- Omobuwajo, O. R., Abdu, A., Igbeneghu, O. A., Agboola, I. O. & Alade, G. O. (2011). Preliminary investigation of an herbal soap incorporating Cassia senna(L), Roxby leaves and Ageratum conyzoidesLinn whole plant powders. Journal of Herbal Sciences, 21; 15- 17.
- Rama, S, Arya, M., Arya, P., Biswas, D. & Prasad, R., (2011). Antimicrobial susceptibility pattern of bacterial isolates from post-operative wound infections. Indian Journal of Pathology and Microbiology, 48(2), 266–269.
- Riaz T.C., Kilani, A. M. Garner O. O. & Adeleke, O. E. (2019). Antibacterial effect of soaps against daily encountered bacteria in Kigali. African Journal of Biotechnology, 6(22):2529- 2431.
- Saba, R., Adeel, H. & Shahida, H. (2019). Antibacterial Activity of Soaps against daily encountered bacteria. African Journal of Biotechnology, 8: 1431-1436.
- Selvamohan, V. & Sandhya, T. (2012). Studies on the bactericidal activity of different soaps against bacterial strains. Journal of Microbiology and Biotechnology Research,(5): 646- 650.
- Sorescu, I. and Stoica, C. (2021). Online advanced bacterial identification software, an original tool for phenotypic bacterial identification. Romanian Biotechnological letters, 26(6): 3047-3053
- Tachibana, D. K. (2016). Reviews of locally made soup and industrial soups. Journal annual review of microbiology. 30: 350-375.
Soaps are recognized primarily as cleaning agents due to their antibacterial properties. The antibacterial efficacy of
four distinct soaps, specifically a beauty soap, medicated soap, black soap, and local soap, was examined. Standard
microbiological procedures, were employed for the identification of the isolates. The disc diffusion assay was employed to
investigate the antibacterial efficacy of the soap samples against the isolates at various concentrations: 200 mg/ml, 100 mg/ml,
50 mg/ml, 25 mg/ml, and 12.5 mg/ml. The bacteria were identified as Escherichia coli, Staphylococcus sp., and Bacillus sp. At a
concentration of 200 mg/ml, local soap inhibited the growth of all isolates, with the maximum inhibition measuring 31 mm and
the minimum inhibition measuring 8.0 mm. At a concentration of 100 mg/ml, black soap and local soap exhibited significant
inhibition against all isolates, with the zone of inhibition decreasing as the concentration of the soap samples decreased. Beauty
soap shown antibacterial activity at concentrations of 100 mg/ml and 50 mg/ml. The minimum inhibitory concentrations (MIC)
for the isolates varied between 12.5 mg/ml and 25 mg/ml, whereas the minimum bactericidal concentrations (MBC) for black
soap and local soap were determined to be 50 mg/ml and 25 mg/ml, respectively, against the isolates. Black soap and local soap,
exhibiting superior efficacy at low concentrations, may be regarded as effective antibacterial agents for cleansing purposes. The
evaluation of antimicrobial efficacy for all four soaps indicated that black soap and Local soap had superior effectiveness against
all isolates in comparison to medicated and beauty soap.
Keywords :
Medicated, Soap, Organic, Microorganism, Skin.