Authors :
Akuaka, G. O; Akawo, N. O; Osagiede, L. A; Odita, N. G
Volume/Issue :
Volume 7 - 2022, Issue 8 - August
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3enVWJC
DOI :
https://doi.org/10.5281/zenodo.7045319
Abstract :
Cellulose is considered to be the most
abundant renewable lignocellulosic material on earth.
The technique employed for the conversion of these
agricultural residues to simple sugars by cellulases thus
has a great industrial and commercial potential and can
open new application fields in the areas of industrial
processes. The process of this conversion of cellulosic
materials by cellulase which is a hydrolytic enzyme was
studied using solid-state fermentation (SSF) and
agricultural wastes as substrates and microorganisms
(Aspergillus niger)in a controlled environment while
giving the organism free access to water molecules. The
fungal organism used was isolated from the area where
the bi-products were obtained. The microbe (A.niger)
was subjugated for the production of an enzyme
carboxymethyl cellulase in a CMC solution by the DNSA
method ata varying range of temperatures and other
varying parameters were studied as well such as the pH,
Carbon and nitrogen sources and effects of metals ion
concentration on the enzyme. It was analyzed using the
Lineweaver-Burk plot which offered a practical
graphical method for analyses of the Michaelis-Menten
equation to evaluate the imperative terms in enzyme
kinetics such as Km and Vmax. The yield was also
compared using the aforementioned equation and
kinetics and it was observed that the enzymatic yield was
higher on rice bran than on corn cob. The enzyme was
tolerant and stable at a varying range of temperatures,
as such is a viable tool in industrial processes and hence
can provide veritable potential in developing
andspurring the economy of the country if well
harnessed.
Keywords :
cellulose, Aspergillus niger, fermentation, substrates, synthesis
Cellulose is considered to be the most
abundant renewable lignocellulosic material on earth.
The technique employed for the conversion of these
agricultural residues to simple sugars by cellulases thus
has a great industrial and commercial potential and can
open new application fields in the areas of industrial
processes. The process of this conversion of cellulosic
materials by cellulase which is a hydrolytic enzyme was
studied using solid-state fermentation (SSF) and
agricultural wastes as substrates and microorganisms
(Aspergillus niger)in a controlled environment while
giving the organism free access to water molecules. The
fungal organism used was isolated from the area where
the bi-products were obtained. The microbe (A.niger)
was subjugated for the production of an enzyme
carboxymethyl cellulase in a CMC solution by the DNSA
method ata varying range of temperatures and other
varying parameters were studied as well such as the pH,
Carbon and nitrogen sources and effects of metals ion
concentration on the enzyme. It was analyzed using the
Lineweaver-Burk plot which offered a practical
graphical method for analyses of the Michaelis-Menten
equation to evaluate the imperative terms in enzyme
kinetics such as Km and Vmax. The yield was also
compared using the aforementioned equation and
kinetics and it was observed that the enzymatic yield was
higher on rice bran than on corn cob. The enzyme was
tolerant and stable at a varying range of temperatures,
as such is a viable tool in industrial processes and hence
can provide veritable potential in developing
andspurring the economy of the country if well
harnessed.
Keywords :
cellulose, Aspergillus niger, fermentation, substrates, synthesis
