Authors :
Musa, J; Danladi, Y. K; Obaroh, I. O; Bandiya, H. M; Sani K. A
Volume/Issue :
Volume 7 - 2022, Issue 4 - April
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3PW6m1r
DOI :
https://doi.org/10.5281/zenodo.6605017
Abstract :
Trypanosomiasis is virulent, inoculable and
highly fatal protozoan disease that affects both human
and domestic animal. The effects of some neem leaf
phytochemicals on weight and Packed Cell Volume
(PCV) of Trypanosoma brucei brucei infected albino rats
have been investigated in this study. Neem leaves
preparation, phytochemicals extraction were done using
standard protocols. Experimental animals and Test
Organism (Trypanosoma brucei brucei) used in this study
were obtained from Nigeria Institute for
Trypanosomiasis Research, (NIFTR) Kaduna. Albino
rats were inoculated with the parasites and screened for
development of infection using the buffy coat technique.
Thirty two rats were randomly distributed into 8 groups.
Group A infected but no treatment; Group B infected
and treated with Diminazine aceturate. Group C to K
were infected and treated with varying concentration
(100, 200 and 400 mg/kg body weight) of either alkaloids,
glycoside neem extract or both. All groups were treated
for 4 days, and given food and water of equal
proportion. Body weights and packed cell volume (PCV)
were determined using standard procedure. The study
revealed that albino rats in the untreated control group
and those administered with 100mg/kg/bodyweight of
either Alkaloids, Glycosides, or both extract of neem
leaves showed gradual decrease in body weight. While
T. b. brucei infected albino rats on extract inoculums of
200 and 400mg/kg/bodyweight and standard drug
showed increase in body weight. The results shows
significant difference (p<0.05) in PCV values between
those in the treatment groups when compared with
group administered with standard drug. There is need
for continuous studies in order to extract and purify
more compounds responsible for the antitrypanosomal
activity of the studied plant.
Keywords :
Trypanosomiasis; Neem; Phytochemicals; Albino rats; Weight and PCV
Trypanosomiasis is virulent, inoculable and
highly fatal protozoan disease that affects both human
and domestic animal. The effects of some neem leaf
phytochemicals on weight and Packed Cell Volume
(PCV) of Trypanosoma brucei brucei infected albino rats
have been investigated in this study. Neem leaves
preparation, phytochemicals extraction were done using
standard protocols. Experimental animals and Test
Organism (Trypanosoma brucei brucei) used in this study
were obtained from Nigeria Institute for
Trypanosomiasis Research, (NIFTR) Kaduna. Albino
rats were inoculated with the parasites and screened for
development of infection using the buffy coat technique.
Thirty two rats were randomly distributed into 8 groups.
Group A infected but no treatment; Group B infected
and treated with Diminazine aceturate. Group C to K
were infected and treated with varying concentration
(100, 200 and 400 mg/kg body weight) of either alkaloids,
glycoside neem extract or both. All groups were treated
for 4 days, and given food and water of equal
proportion. Body weights and packed cell volume (PCV)
were determined using standard procedure. The study
revealed that albino rats in the untreated control group
and those administered with 100mg/kg/bodyweight of
either Alkaloids, Glycosides, or both extract of neem
leaves showed gradual decrease in body weight. While
T. b. brucei infected albino rats on extract inoculums of
200 and 400mg/kg/bodyweight and standard drug
showed increase in body weight. The results shows
significant difference (p<0.05) in PCV values between
those in the treatment groups when compared with
group administered with standard drug. There is need
for continuous studies in order to extract and purify
more compounds responsible for the antitrypanosomal
activity of the studied plant.
Keywords :
Trypanosomiasis; Neem; Phytochemicals; Albino rats; Weight and PCV
