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Dual Fungal Inoculation Accelerates Degradation Kinetics and Enhances Compost Maturity in Sterilized Sugarcane Bagasse


Authors : Nabaroon Das; Dr. Adlene Sangeeth

Volume/Issue : Volume 11 - 2026, Issue 6 - June


Google Scholar : https://tinyurl.com/434nm9mk

Scribd : https://tinyurl.com/mrx5uk33

DOI : https://doi.org/10.38124/ijisrt/26jun907

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : Sugarcane bagasse is one of the most abundantly produced lignocellulosic agricultural residues globally, yet its high lignin content and characteristically elevated carbon-to-nitrogen (C:N) ratio—often exceeding 60:1—present substantial barriers to efficient microbial decomposition and timely compost stabilization. While previous research has largely pursued environmental optimization or single-enzyme interventions as strategies to accelerate composting, the potential of deliberately engineered fungal consortia to improve both degradation kinetics and compost quality remains poorly characterized. We hypothesized that the simultaneous introduction of Phanerochaete chrysosporium and Trichoderma viride—two fungi with complementary but non-overlapping substrate specificities—would generate synergistic improvements in both the rate of organic carbon mineralization and final compost maturity relative to either fungus inoculated alone. To test this, sterilized sugarcane bagasse was inoculated under controlled static conditions at 30°C, with measurements of organic carbon, lignin, total nitrogen, C:N ratio, phenolic content, and the germination index recorded at Day 0, Day 30, and Day 60.

Keywords : Sugarcane Bagasse; Lignocellulose; Composting; Fungal Consortium; Degradation Kinetics; Compost Maturity.

References :

  • Microbial Biodecomposition & Fungal Reviews
  1. Konan, D., Kouamé, K. A. J., Amon, A. S., & Tano, K. (2024). Biodecomposition with Phanerochaete chrysosporium: A review. AIMS Microbiology, 10(4), 1068–1101. https://doi.org/10.3934/microbiol.2024046
  2. De Medina-Salas, L., Castillo-González, E., & Giraldi-Díaz, M. R. (2021). Effect of microbial inoculation on nutrient turnover and lignocellulose degradation during composting: A meta-analysis. Waste Management, 125, 220–232. https://doi.org/10.1016/j.wasman.2021.03.007
  • Cellulase Production & Kinetics
  1. Nathan, V. K., Kanth, S. V., & Devi, C. S. (2014). Process optimization and production kinetics for cellulase production by Trichoderma viride VKF3. SpringerPlus, 3, 92. https://doi.org/10.1186/2193-1801-3-92
  2. Puthumana, A. B., & Kaparaju, P. (2024). Impact of organic load on methane yields and kinetics during anaerobic digestion of sugarcane bagasse: Optimal feed-to-inoculum ratio and total solids of reactor working volume. Energies, 17(20), 5083. https://doi.org/10.3390/en17205083
  • Agricultural Waste Management & Composting
  1. Alburquerque, J. A., Gonzálvez, J., García, D., & Cegarra, J. (2006). Measuring detoxification and maturity in compost made from "Alperujo", the solid by-product of extracting olive oil by the two-phase centrifugation system. Chemosphere, 64(3), 470–477. https://doi.org/10.1016/j.chemosphere.2005.10.055
  2. Organo, N. D., Granali, E. L., de los Santos, G. P., & Migo, V. P. (2022). Assessing the potential of a Trichoderma-based compost activator to hasten the decomposition of incorporated rice straw. Scientific Reports, 12, 1398. https://doi.org/10.1038/s41598-021-03828-1
  3. Wagner, A. O., Lins, P., Illmer, P., & Markt, R. (2016). Improving green waste composting by addition of sugarcane bagasse and exhausted grape marc. Journal of Environmental Management, 182, 557–563. https://doi.org/10.1016/j.jenvman.2016.07.062

Sugarcane bagasse is one of the most abundantly produced lignocellulosic agricultural residues globally, yet its high lignin content and characteristically elevated carbon-to-nitrogen (C:N) ratio—often exceeding 60:1—present substantial barriers to efficient microbial decomposition and timely compost stabilization. While previous research has largely pursued environmental optimization or single-enzyme interventions as strategies to accelerate composting, the potential of deliberately engineered fungal consortia to improve both degradation kinetics and compost quality remains poorly characterized. We hypothesized that the simultaneous introduction of Phanerochaete chrysosporium and Trichoderma viride—two fungi with complementary but non-overlapping substrate specificities—would generate synergistic improvements in both the rate of organic carbon mineralization and final compost maturity relative to either fungus inoculated alone. To test this, sterilized sugarcane bagasse was inoculated under controlled static conditions at 30°C, with measurements of organic carbon, lignin, total nitrogen, C:N ratio, phenolic content, and the germination index recorded at Day 0, Day 30, and Day 60.

Keywords : Sugarcane Bagasse; Lignocellulose; Composting; Fungal Consortium; Degradation Kinetics; Compost Maturity.

Paper Submission Last Date
30 - June - 2026

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