Authors : Motiki F. Beleme; Lebohang A. Moetseloa; Letuka J. Sello

Volume/Issue : Volume 9 - 2024, Issue 8 - August

Google Scholar : https://tinyurl.com/28b9yed5

Scribd : https://tinyurl.com/y8a6afd4

DOI : https://doi.org/10.38124/ijisrt/IJISRT24AUG262

Abstract : A simple spectrophotometric technique has been developed and validated for determination of betamethasone 17-valerate in pharmaceutical formulations. The method is based on cream dissolution in absolute ethanol and assaying spectrophotometrically at 240nm. The amount of betamethasone 17-valerate in cream was determined as 101.6 ± 0.0037 % w/w. The method validation demonstrated linearity for concentration range of 0.006mg/ml to 0.0014mg/ml (R2=0.9995). The method is simple and accurate and has successfully been employed in the analysis of some of Tripharm pharmaceutical formulations.

Keywords : Betamethasone 17-Valerate, Spectrophotometry, Betamethasone, HPLC.

References :

1. Asra, R., Rivai, H. and Astuty, W. (2017) ‘Pengembangan dan Validasi Metode Analisis Betametason Tablet dengan Metode Absorbansi dan Luas Daerah di Bawah Kurva Secara Spektrofotometri Ultraviolet’, Jurnal Farmasi Higea, 9(2), pp. 118–126.
2. Awen, B. et al. (2010) ‘2. Development and validation of a new spectrophotometric method for the determination of betamethasone in bulk and pharmaceutical dosage forms’, International Journal of Pharmaceutical and Biomedical Research (IJPBR) [Preprint].
3. Deceuninck, Y. et al. (2011) ‘Determination of MRL regulated corticosteroids in liver from various species using ultra high performance liquid chromatography–tandem mass spectrometry (UHPLC)’, Analytica chimica acta, 700(1–2), pp. 137–143.
4. Gupta, M.K. et al. (2022) ‘A comparative review on High-Performance Liquid Chromatography (HPLC), Ultra Performance Liquid Chromatography (UPLC) & High-Performance Thin Layer Chromatography (HPTLC) with current updates’, Current Issues in Pharmacy and Medical Sciences [Preprint].
5. Nikolin, B. et al. (2004) ‘High perfomance liquid chromatography in pharmaceutical analyses’, Bosnian journal of basic medical sciences, 4(2), p. 5.
6. Permata, Y. et al. (2019) ‘Formulation and Quantitative Analysis of Betamethasone Valerate and Neomycin Sulfate Cream by High Performance Liquid Chromatography and Spectrophotometry’, Open Access Macedonian Journal of Medical Sciences, 7, pp. 3841–3846. Available at: https://doi.org/10.3889/oamjms.2019.516.
7. da Silva Solon, L.G. et al. (2016) ‘Development and validation of an UHPLC method for the determination of betamethasone valerate in cream, gel, ointment and lotion’, Steroids, 106, pp. 70–77.
8. Smith, E.W., Haigh, J.M. and Kanfer, I. (1985) ‘A stability-indicating HPLC assay with on-line clean-up for betamethasone 17-valerate in topical dosage forms’, International journal of pharmaceutics, 27(2–3), pp. 185–192.
9. Walfish, S. (2006) ‘Analytical methods: a statistical perspective on the ICH Q2A and Q2B guidelines for validation of analytical methods’, BioPharm International, 19(12), pp. 1–6.