A new and economical UV/vis
spectrophotometric method using 0.1M hydrochloric
acid as a dilution solvent for the estimation of vitamin C
pure and in its dosage forms was developed in this work.
The linearity study was performed over a range of
concentrations from 2 to 12 µg / ml. The maximum
absorption wavelength found was 243 nm. Linearity,
limit of detection (LOD), limit of quantification (LOQ),
precision, accuracy, specificity / selectivity and
robustness were chosen as criteria for validation of the
method according to the requirements of the
International Conference of 'Harmonization (ICH). The
developed method was used to quantitatively determine
vitamin C in its pharmaceutical forms marketed on the
local market in the City of Kinshasa, DR Congo. The
results obtained in this research demonstrate that the
method is exact, precise, reproducible (% RSD <2%),
simple, sensitive, robust, selective / specific and rapid.
Keywords : Ascorbic Acid, Spectrophotometry, UV/vis, Development, Validation.