Development and Validation of a UV- Visible Method for Determining Piperine in Bulk and Formulation


Authors : Dr. Sapna Shrikumar; Ardhra Maria M F; Gadha M; Gayathri P; Gopika Praveen; Jisha K

Volume/Issue : Volume 9 - 2024, Issue 7 - July

Google Scholar : https://tinyurl.com/3bv34p7f

Scribd : https://tinyurl.com/k8z92rzy

DOI : https://doi.org/10.38124/ijisrt/IJISRT24JUL952

Abstract : Standardization of herbal preparations is crucial because the growing use of herbal medicines and the globalization of the herbal medicinal industries have raised serious concerns about safety among the public and health authorities. However, the majority of the time, it was discovered that the standardization procedure was challenging and time-consuming. Thus, piperine from Piper nigrum in the commercial formulation could be quantitatively determined and standardized using an easy-to-use and trustworthy UV-Visible Spectrophotometric technique. In accordance with WHO guidelines, UV-visible spectrophotometric investigations of several parameters are part of the study. This work presents a spectrophotometric technique in the UV area at 342 nm that is easy to use, quick, sensitive and accurate. It was established how much piperine was present in the sample and validated. Therefore, piperine in the commercial formulations may be reliably quantified using the suggested method.

Keywords : Standardization, UV-Visible Spectroscopy, Piperine, Piper Nigrum.

References :

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  11. Chethan Kumar H B, Bhaskar Kurangi, Moazzim Soudagar, Supriya Chimgave, Swapnil Patil; Development and Validation of UV-Spectrophotometric Method for Estimation of Vinpocetine in Marketed Formulation and Nanoformulation; International Journal of Ayurvedic Medicine, Vol 14 (3), 2023; 717-723.
  12. Kollol Kumar Majumder, Jai Bharti Sharma, Manish Kumar, Shailendra Bhatt, Vipin Saini; Development and validation of uv-visible spectrophotometric method for the estimation of curcumin in bulk and pharmaceutical formulation; Pharmacophore, 11(1) 2020, Pages:115-121
  13. Dr. Sapna Shrikumar and Akshaya Unni K C, “Averrhoa carambola: an overview”, World journal of pharmaceutical research, 2024, Vol 13, Issue 10.
  14. Dr. Sapna Shrikumar, Asish S, Akshaya S, Jesvin Joby and Sreelakshmi K “Pharmacognostical and phytochemical studies for the standardisation of plant Nycanthes arbor-tristis Linn” International Journal of Green and Herbal Chemistry, June 2024, Vol 13, Issue 3, Pg 234-243.
  15.  Dr. Sapna Shrikumar, Afra P, Fathimathul Nasrin K A and Mufeeda “Standardisation of Pouteria campechiana leaves for its pharmacognostical and phytochemical studies” African Journal of Biological Sciences, June 2024, Vol 6, Issue 5, Pg 6312-6326.
  16. Dr. Sapna Shrikumar, Maria Nison, Nanditha Das P M and Sreeshma K Babu “Pharmacognostical and Phytochemical studies on leaves of Gliricidia sepium Kunth”, International Journal of Green and Herbal Chemistry, June 2024, Vol 13, Issue 3, Pg 219-233.
  17. Dr. Sapna Shrikumar and Archana.S “Exploring the therapeutic potential of Clerodendrum infortunatum: A comprehensive review” International Journal of Creative Research Thoughts (IJCRT), June 2024, Vol 12, Issue 6, Pg 699-706.
  18. Dr. Sapna Shrikumar, Drisya M K and Surya S Nair “Review on Essential oil: Antimicrobial activities of Origanum manjoram and Thymus vulgaris” World Journal of Pharmaceutical Research, June 2024, Vol 13, Issue 11, Pg 248-256

Standardization of herbal preparations is crucial because the growing use of herbal medicines and the globalization of the herbal medicinal industries have raised serious concerns about safety among the public and health authorities. However, the majority of the time, it was discovered that the standardization procedure was challenging and time-consuming. Thus, piperine from Piper nigrum in the commercial formulation could be quantitatively determined and standardized using an easy-to-use and trustworthy UV-Visible Spectrophotometric technique. In accordance with WHO guidelines, UV-visible spectrophotometric investigations of several parameters are part of the study. This work presents a spectrophotometric technique in the UV area at 342 nm that is easy to use, quick, sensitive and accurate. It was established how much piperine was present in the sample and validated. Therefore, piperine in the commercial formulations may be reliably quantified using the suggested method.

Keywords : Standardization, UV-Visible Spectroscopy, Piperine, Piper Nigrum.

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