Authors : Ahsaan Ullah; Muhammad Saqib

Volume/Issue : Volume 8 - 2023, Issue 6 - June

Google Scholar : https://bit.ly/3TmGbDi

Scribd : https://tinyurl.com/yc68e7cv

DOI : https://doi.org/10.5281/zenodo.8195499

Abstract : “Theileria parva” (TP), an “apicomplexan parasite” that causes “East Coast Fever” (ECF) in cattle, in Pakistan. East coast fever (ECF) is a disease spread by ticks that is thought to cause at least $300 million in annual economic losses. The production procedure & other facets of the vaccine have not been sufficiently standardised, despite the fact that it is the only commercially available vaccination for this lethal cattle illness. Notably, the “Pakistan Veterinary Research Institute (PVRI)” produced its most recent production batch at the beginning of 2022. A pathogen-free tick colony and parasite-free cattle are used in the manufacturing of this vaccine, and both are closely watched during the entire procedure. This article describes the procedure used in the most current Infection and Treatment Method (ITM) vaccine production and introduces process improvements that are not present in earlier production versions. These upgrades include improved quality control measures. The report also describes how a three-phase in-vivo investigation with different vaccine stability dilutions are used to establish the ideal field dose. After post-production evaluations verified the vaccine's viability and safety, it is decided that a field dosage of one millilitre (ml) at a 1:100 weakening would be appropriate.

Keywords : Theileria parva; East Coast Fever; Pakistan Veterinary Research Institute; Vaccine.