Authors :
Bandi Balaobulapathi; Sudhakar Podha; Dr. Nagarajan; Srikanth Adabala; Abhinay Gontu
Volume/Issue :
Volume 8 - 2023, Issue 12 - December
Google Scholar :
https://tinyurl.com/4x3kk67d
Scribd :
https://tinyurl.com/yc86tm9e
DOI :
https://doi.org/10.5281/zenodo.10298353
Abstract :
Chikungunya virus was purified with Cesium
chloride (CsCl) density gradients by ultracentrifugation
from the cell culture supernatant of infected Vero cell.
The purification process was done by using combinations
of CsCl and PBS (Phosphate buffer saline) and the virus
band was found at a density of 1.00 g/ml after
centrifugation for 3 hr of ultracentrifugation. The
authenticity of the purified Chikungunya virus was
confirmed by SDS PAGE. The Purification process has
immense application and is useful in making large
quantity of purified Chikungunya virus with high titer
values for its characterization.
Keywords :
Chikungunya Virus, Centrifugation, Vero Cells, CsCl, TFF, Antigen.
Chikungunya virus was purified with Cesium
chloride (CsCl) density gradients by ultracentrifugation
from the cell culture supernatant of infected Vero cell.
The purification process was done by using combinations
of CsCl and PBS (Phosphate buffer saline) and the virus
band was found at a density of 1.00 g/ml after
centrifugation for 3 hr of ultracentrifugation. The
authenticity of the purified Chikungunya virus was
confirmed by SDS PAGE. The Purification process has
immense application and is useful in making large
quantity of purified Chikungunya virus with high titer
values for its characterization.
Keywords :
Chikungunya Virus, Centrifugation, Vero Cells, CsCl, TFF, Antigen.
