Authors :
Dr. Subhashini S.; Dr. Sachin; Dr. Sharath Kumar Shetty B.; Dr. Mahesh kumar Y.
Volume/Issue :
Volume 11 - 2026, Issue 8 - August
Google Scholar :
https://tinyurl.com/5n7fhmwb
DOI :
https://doi.org/10.38124/ijisrt/26aug1316
Note : A published paper may take 4-5
working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and
ResearchGate.
Abstract :
Introduction:
Clear aligner therapy offers an aesthetic and removable alternative to conventional orthodontic appliances. However,
aligners are continuously exposed to the oral environment and may undergo changes in surface characteristics, colour and
biological contamination during clinical use. Appropriate cleaning is therefore important for maintaining aligner integrity
and hygiene. The present study evaluated the effects of commonly used patient-performed cleaning methods on clear
orthodontic aligners.
Aim:
To compare the effects of five cleaning methods on the surface roughness, colour change and ATP bioluminescence of
clear orthodontic aligners following 14 days of intraoral use.
Materials and Methods:
An in-vivo comparative study was conducted among 60 participants, randomly allocated into five groups of 12 each:
soap solution, Orodox cleansing tablets, Toothsi cleaning foam, water (control), and baking soda paste. Each aligner was
used for 14 days and cleaned daily according to the assigned protocol. Surface roughness was evaluated using a surface
profilometer and expressed as Ra (µm). Colour change was assessed using a spectrophotometer and expressed as ΔE.
Biological contamination was assessed using an ATP bioluminometer and expressed as relative light units (RLU). Data were
analysed using IBM SPSS Statistics. One-way ANOVA followed by Tukey's HSD post-hoc test was used for surface
roughness and colour change. Since the homogeneity of variance assumption was violated for ATP, Welch's ANOVA
followed by Games–Howell post-hoc analysis was performed. A p-value <0.05 was considered statistically significant.
Keywords :
Clear Aligners; Aligner Cleaning; Surface Roughness; Colour Stability; ATP Bioluminescence; Orthodontic Aligners; Orodox; Toothsi; Baking Soda; Oral Hygiene.
References :
- Phan X, Ling PH. Clinical limitations of Invisalign. J Can Dent Assoc. 2007;73:263-266.
- Kravitz ND, Kusnoto B, Begole E, Obrez A, Agran B. How well does Invisalign work? A prospective clinical study evaluating the efficacy of tooth movement with Invisalign. Am J Orthod Dentofacial Orthop. 2009;135:27-35.
- Weir T. Clear aligners in orthodontic treatment. Aust Dent J. 2017;62:58-62.
- Eliades T, Zinelis S, Eliades G, Athanasiou AE. Characterization of orthodontic thermoplastic materials. Eur J Orthod. 2007;29:200-205.
- Zhang N, Bai Y, Ding X, Zhang Y. Preparation and characterization of thermoplastic materials for invisible orthodontics. Dent Mater J. 2011;30:954-959.
- Eliades T, Bradley TG, Zinelis S, Eliades G. Surface texture changes of orthodontic materials subjected to clinical aging. Eur J Orthod. 2014;36:522-528.
- Levrini L, Mangano A, Margherini S, Tenconi C, Vigetti D, Muollo R, et al. ATP bioluminometer analysis on the surfaces of removable orthodontic aligners after the use of different cleaning methods. Int J Dent. 2016;2016:5926941.
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- Lombardo L, Martines E, Mazzanti V, Arreghini A, Mollica F, Siciliani G. Stress relaxation properties of four orthodontic aligner materials: a 24-hour in vitro study. Angle Orthod. 2017;87:11-18.
- Papadopoulos MA, Eliades T. Aging of orthodontic materials: biological and mechanical considerations. Semin Orthod. 2018;24:123-132.
- Papadopoulou AK, Cantele A, Polychronis G, Zinelis S, Eliades T. Changes in roughness and mechanical properties of Invisalign appliances after clinical use. Am J Orthod Dentofacial Orthop. 2019;155:44-55.
- Iliadi A, Enzler V, Polychronis G, Peltomäki T, Zinelis S, Eliades T. Effect of cleansers on the composition and mechanical properties of orthodontic aligners in vitro. Prog Orthod. 2022;23:54.
- Alweneen A, Alqahtani N. The effectiveness of different cleaning methods for clear orthodontic aligners: impacts on physical, mechanical, and chemical properties—an in vivo study. Polymers (Basel). 2025;17:1620.
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- Condò R, Mampieri G, Giancotti A, Cerroni L, Pasquantonio G, Divizia A, et al. SEM characterization and ageing analysis on two generation of invisible aligners. BMC Oral Health. 2021;21:316.
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Introduction:
Clear aligner therapy offers an aesthetic and removable alternative to conventional orthodontic appliances. However,
aligners are continuously exposed to the oral environment and may undergo changes in surface characteristics, colour and
biological contamination during clinical use. Appropriate cleaning is therefore important for maintaining aligner integrity
and hygiene. The present study evaluated the effects of commonly used patient-performed cleaning methods on clear
orthodontic aligners.
Aim:
To compare the effects of five cleaning methods on the surface roughness, colour change and ATP bioluminescence of
clear orthodontic aligners following 14 days of intraoral use.
Materials and Methods:
An in-vivo comparative study was conducted among 60 participants, randomly allocated into five groups of 12 each:
soap solution, Orodox cleansing tablets, Toothsi cleaning foam, water (control), and baking soda paste. Each aligner was
used for 14 days and cleaned daily according to the assigned protocol. Surface roughness was evaluated using a surface
profilometer and expressed as Ra (µm). Colour change was assessed using a spectrophotometer and expressed as ΔE.
Biological contamination was assessed using an ATP bioluminometer and expressed as relative light units (RLU). Data were
analysed using IBM SPSS Statistics. One-way ANOVA followed by Tukey's HSD post-hoc test was used for surface
roughness and colour change. Since the homogeneity of variance assumption was violated for ATP, Welch's ANOVA
followed by Games–Howell post-hoc analysis was performed. A p-value <0.05 was considered statistically significant.
Keywords :
Clear Aligners; Aligner Cleaning; Surface Roughness; Colour Stability; ATP Bioluminescence; Orthodontic Aligners; Orodox; Toothsi; Baking Soda; Oral Hygiene.