Tuberculosis is a global problem and incidence of
extra pulmonary tuberculosis is increasing day by day.
Diagnosis of extra pulmonary tuberculosis is a little bit
difficult than pulmonary tuberculosis.Aim and Objective:
The aim of this study is to correlate between
isolates of mycobacterium in GeneXpert and ZiehlNeelsen (ZN) staining. It also detects the sensitivity,
specificity, positive predictive value (PPV) and negative
predictive value (NPV) of GeneXpert assay and ZN
staining.This prospective study was carried out in the
Department of Microbiology, RIMS, Ranchi,
Jharkhand. Seventy two samples from suspected
patients of Extra pulmonary tuberculosis were taken.
These samples were processed for test in GeneXpert
assay, ZN staining and MGIT culture. Mycobacterium
tuberculosis complex isolated from culture was taken as
gold standard and compared with result of GeneXpert
and ZN staining. Result: Out of 72 samples, detection
rate of GeneXpert, MGIT and ZN staining were
27.77%, 26.38% and 18%. The sensitivity, specificity,
PPV and NPV of ZN staining and GeneXpert were
63.15%, 98.11%, 92.3%, 88.13% and 94.73%, 96.22%,
90%, 98% respectively.
GeneXpert is a rapid and easy method for extra
pulmonary tuberculosis diagnosis. It not only detect the
bacilli but also diagnose rifampicin drug resistance.
This method prompts in diagnosis and treatment.
Keywords : Pulmonary Tuberculosis, MGIT 960, GeneXpert MTB/RIF, ZN staining, Acid Fast Bacilli.