Authors : Seema Dixit; Nisha Uraiti

Volume/Issue : Volume 10 - 2025, Issue 11 - November

Google Scholar : https://tinyurl.com/mpbfhnxf

Scribd : https://tinyurl.com/jcsp5jpk

DOI : https://doi.org/10.38124/ijisrt/25nov1527

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Abstract : Investigation of antioxidant properties of Trigonella foenum-graecum (fenugreek) seed and Hibiscus rosa-sinensis (China rose) bark extracts in phenylhydrazine (PHZ)-induced anaemia models in mice. Ethanol extracts of both plant materials were prepared through maceration and evaluated for in-vitro antioxidant activity using DPPH radical scavenging, hydrogen peroxide scavenging, and reducing power assays. Results revealed dose-dependent antioxidant activity, with Hibiscus rosa-sinensis showing greater reducing power and Trigonella foenum-graecum demonstrating superior hydrogen peroxide and DPPH scavenging activity. In vivo, anaemia was induced in Swiss albino mice using PHZ (10 mg/kg body weight), leading to significant reductions in antioxidant enzyme levels including superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GSHPx). Treatment with individual and combined plant extracts significantly restored antioxidant parameters. Acute and sub-acute toxicity studies established the safety of both extracts up to 2000 mg/kg, and a combined dose of 400 mg/kg body weight was found effective and non-toxic. These findings support the therapeutic potential of T. foenum-graecum and H. rosa-sinensis as natural antioxidants with haemoprotective efficacy, particularly in oxidative stress-related anaemic conditions.

Keywords : Trigonella foenum-graecum, Hibiscus rosa-sinensis, Antioxidant Activity, Phenylhydrazine, Anaemia, Mice Model.

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