Authors :
Shivani Dipakbhai Patel; Dr. Aditee Pandya
Volume/Issue :
Volume 7 - 2022, Issue 5 - May
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3NeZBq5
Abstract :
Tinospora cordifolia is a priority medicinal
plant with significant therapeutic value, particularly in
Indian medicine. However, for quick multiplication, this
plant requires a regeneration technique. T. cordifolia
nodal explants were used to design a successful
regeneration technique.When nodal segments were
cultivated on MS media enriched with BAP and 2-iP, a
high frequency of multiple shoot development was
produced. On this medium and hormonal concentration,
the maximum mean number of shoots per nodal explant,
the longest shoot length, and an 86 percent response
were attained. Tinospora cordifolia (Willd) Miers has a
successful in vitro micropropagation strategy based on
shoot tip explants. Young and mature shoot tip (YST
and MST) explants from two different-aged plant
sources (15 days and 3 years) were treated
simultaneously in the micropropagation methodology to
develop and evaluate their effectiveness.6-benzyladenine
(BA) and kinetin (KN) performed better in bud break
and shoot growth than the other cytokinins and
synergetic treatments, at both individual and combined
dosages.Multiple shoot induction and development were
improved when the auxin indole-3-acetic acid (IAA) was
added to the appropriate cytokinin doses of BA and
KN.Additional gibberellic acid and an antioxidant
(ascorbic acid) supplementation improved shoot bud
induction, shot number, and shoot length
considerably.Also, this review article gives an idea of
application of Tinosporacordifolia and how to enhanced
micropropagation by the combination of various Plant
Growth Regulators and its concentration.
Tinospora cordifolia is a priority medicinal
plant with significant therapeutic value, particularly in
Indian medicine. However, for quick multiplication, this
plant requires a regeneration technique. T. cordifolia
nodal explants were used to design a successful
regeneration technique.When nodal segments were
cultivated on MS media enriched with BAP and 2-iP, a
high frequency of multiple shoot development was
produced. On this medium and hormonal concentration,
the maximum mean number of shoots per nodal explant,
the longest shoot length, and an 86 percent response
were attained. Tinospora cordifolia (Willd) Miers has a
successful in vitro micropropagation strategy based on
shoot tip explants. Young and mature shoot tip (YST
and MST) explants from two different-aged plant
sources (15 days and 3 years) were treated
simultaneously in the micropropagation methodology to
develop and evaluate their effectiveness.6-benzyladenine
(BA) and kinetin (KN) performed better in bud break
and shoot growth than the other cytokinins and
synergetic treatments, at both individual and combined
dosages.Multiple shoot induction and development were
improved when the auxin indole-3-acetic acid (IAA) was
added to the appropriate cytokinin doses of BA and
KN.Additional gibberellic acid and an antioxidant
(ascorbic acid) supplementation improved shoot bud
induction, shot number, and shoot length
considerably.Also, this review article gives an idea of
application of Tinosporacordifolia and how to enhanced
micropropagation by the combination of various Plant
Growth Regulators and its concentration.
