Authors : Dr. Kalpana Pawar; Dr. Komal Potfode; Dr. Sadashiv Daokar; Dr. Shivani Vyavahare; Dr. Shubhankar Nandkhedkar; Dr. Renu Asodekar

Volume/Issue : Volume 8 - 2023, Issue 12 - December

Google Scholar : http://tinyurl.com/e8kctvpv

Scribd : http://tinyurl.com/yvytf75x

DOI : https://doi.org/10.5281/zenodo.10464679

Abstract : Dentin is a critical component in adhesive restorations, and its bonding characteristics differ from those of enamel. Dentin organic tissue that contains collagen fibrils, hydroxyapatite crystals, and water. Unlike enamel, dentin bonding can be more challenging due to its composition and structure. Several factors contribute to the deterioration of dentin bonding over time, such as the presence of water, collagen degradation, and enzymatic activity. Additionally, in clinical situations where caries affected dentin is present, the bond strength may be compromised due to changes in the structure of the dentin. To address these challenges and enhance the durability of adhesive restorations on dentin, researchers and clinicians have explored various techniques, and one of them involves dentin biomodification with collagen cross-linking agents. Collagen cross-linking agents are substances that help stabilize and strengthen the collagen matrix within dentin. By enhancing the integrity of the collagen network, these agents aim to improve the bond strength and overall performance of adhesive restorations. Common collagen cross-linking agents include glutaraldehyde, formaldehyde, and various other chemical agents. These agents work by forming covalent bonds between adjacent collagen molecules, thereby increasing the resistance of the collagen matrix to degradation. It's important to note that while dentin biomodification with collagen cross-linking agents shows promise in improving bond strength and durability, the long-term clinical success and safety of these techniques need further research and validation. Dentistry is a dynamic field, and ongoing research may lead to advancements in adhesive techniques and biomodification strategies for better outcomes in restorative dentistry. This study aimed Compare and evaluate the effect of two collagen cross linking agent cocoa seed extract and Glutaraldehyde on microshear bond strength of caries affected dentin – resin complex.  Materials and Methods  Thirty freshly extracted teeth with caries involving dentin were used. The roots of 30 molars were standardized and embedded in acrylic resin. Enamel was removed from all occlusal surfaces using a diamond saw disk in a high-speed handpiece under copious air-water spray. Acid etching with 37% phosphoric acid gel was performed for 15 seconds, followed by thorough rinsing with water. The samples were divided into two groups:  Group 1 (n = 15): Treated with 6.5% cocoa seed extract.  Group 2 (n = 15): Treated with 5% glutaraldehyde.  Group 1: 6.5% cocoa seed extract was applied on caries-affected dentin for 10 minutes, rinsed with water, and blot dried.  Group 2: 5% glutaraldehyde was applied for 10 minutes and blot dried. Composite was built up with a thickness of 4mm on caries-affected dentin. Each sample was tested under a universal testing machine to measure micro-shear bond strength (μSBS). The recorded data was subjected to statistical analysis.Results and Conclusion  Within the limitations of the study, caries-affected dentin treated with 6.5% cocoa seed extract resulted in increased micro-shear bond strength (μSBS) when compared to 5% glutaraldehyde.