Authors :
Irina Khelisupali
Volume/Issue :
Volume 7 - 2022, Issue 9 - September
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3fguE8Y
DOI :
https://doi.org/10.5281/zenodo.7264291
Abstract :
This study aimed to determine the efficacy of
biological control of the Colorado potato beetle
(Leptinotarsa decemlineata) through Entomopathogenic
nematode (EPNs) Steinernema carpocapsae in both
laboratory and field conditions.
Before starting the experiment, Entomopathogenic
nematodes (EPNs) were isolated from the soil, which were
obtained at different locations of Samtskhe-Javakheti
region, in particular, soil samples were taken for the
experiment from borough Bakuriani, village Timotesubani,
borough Dviri, borough Tsaghveri (from the agricultural
plots of land, various parts of Arjevanidze forest). The
experiments conducted in the laboratory conditions
determined, that all insects participating in the experiment
died, including Imago (95%), while the mortality of larvae
reached 99,5%, which undoubtedly indicates the efficacy of
entomopathogenic nematodes. This experiment showed,
high efficacy of Steinernema carpocapsae towards imagos
and larvae of Colorado potato beetle.
Accordingly, the determination of the efficacy of
Entomopathogenic nematode (EPNs) in the field conditions
became necessary, particularly in the plot of land of the
potato. This experiment also showed, the efficacy of
Steinernema carpocapsae. Almost all Metamorphic forms of
adult Colorado potato beetle and final stage larva died, as a
result of suspension treatment of entomopathogenic
namatodes of potato bush. Of which, adult beetle mortality
reached (75 %), while the mortality of final stage larvas
amounted to 88,5%. As a result of filtrate inspection and
microscopic examination, it was determined, that nematode
suspension showed good results even in field conditions. It
is also noteworthy, that the target insect for
entomopathogenic nematode is only an insect-pest and at
the same time it does not influence the growth and
development of the plant and its fruit (tuber), which is a
quite effective method in terms of ecology, because it does
not require the use of pesticides and other poisonous
chemical products and therefore it is an environmentally
safe natural process.
Keywords :
Entomopathogenic nematode, Steinernema carpocapsae, Leptinotarsa decemlineata
This study aimed to determine the efficacy of
biological control of the Colorado potato beetle
(Leptinotarsa decemlineata) through Entomopathogenic
nematode (EPNs) Steinernema carpocapsae in both
laboratory and field conditions.
Before starting the experiment, Entomopathogenic
nematodes (EPNs) were isolated from the soil, which were
obtained at different locations of Samtskhe-Javakheti
region, in particular, soil samples were taken for the
experiment from borough Bakuriani, village Timotesubani,
borough Dviri, borough Tsaghveri (from the agricultural
plots of land, various parts of Arjevanidze forest). The
experiments conducted in the laboratory conditions
determined, that all insects participating in the experiment
died, including Imago (95%), while the mortality of larvae
reached 99,5%, which undoubtedly indicates the efficacy of
entomopathogenic nematodes. This experiment showed,
high efficacy of Steinernema carpocapsae towards imagos
and larvae of Colorado potato beetle.
Accordingly, the determination of the efficacy of
Entomopathogenic nematode (EPNs) in the field conditions
became necessary, particularly in the plot of land of the
potato. This experiment also showed, the efficacy of
Steinernema carpocapsae. Almost all Metamorphic forms of
adult Colorado potato beetle and final stage larva died, as a
result of suspension treatment of entomopathogenic
namatodes of potato bush. Of which, adult beetle mortality
reached (75 %), while the mortality of final stage larvas
amounted to 88,5%. As a result of filtrate inspection and
microscopic examination, it was determined, that nematode
suspension showed good results even in field conditions. It
is also noteworthy, that the target insect for
entomopathogenic nematode is only an insect-pest and at
the same time it does not influence the growth and
development of the plant and its fruit (tuber), which is a
quite effective method in terms of ecology, because it does
not require the use of pesticides and other poisonous
chemical products and therefore it is an environmentally
safe natural process.
Keywords :
Entomopathogenic nematode, Steinernema carpocapsae, Leptinotarsa decemlineata