Authors :
Shivani Mishra; Shivani Kriti
Volume/Issue :
Volume 8 - 2023, Issue 6 - June
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/mu2am27k
DOI :
https://doi.org/10.5281/zenodo.8084885
Abstract :
CRISPR/Cas9 technology, a genetic
modification tool developed from certain bacteria's
defence mechanism against viruses and plasmids,
brought genome editing back into the public eye in 2012.
This approach is simple to employ and has been tested
on a range of experimental models, including cell lines,
animals in the lab, plants, and even humans in clinical
trials. The Cas9 nuclease is instructed to make a site-
directed double-strand DNA break utilising the short
RNA molecule as a guide in the CRISPR/Cas9 system.
This technology has advanced significantly in recent
years, and it is now often used to edit the genome to add,
remove, or modify certain genes. Additionally,
CRISPR/Cas9-based gene drive may have the ability to
stop the spread of vector-borne illnesses. Although
comprehensive testing is necessary to support these
claims, the bacterial and archaeal adaptive immune
systems may offer a therapeutic solution to previously
incurable illnesses. using regard to disease modelling and
therapy, we discuss current advances made using
CRISPR/Cas9 in this review, as well as what future
considerations should be made when utilising this
technology.
Keywords :
CRISPR/Cas9, Genome editing, Cas proteins, Tumour infiltrating leukocytes (TILs), Nonhomologous end- joining (NHEJ), Homology-directed repair (HDR)
CRISPR/Cas9 technology, a genetic
modification tool developed from certain bacteria's
defence mechanism against viruses and plasmids,
brought genome editing back into the public eye in 2012.
This approach is simple to employ and has been tested
on a range of experimental models, including cell lines,
animals in the lab, plants, and even humans in clinical
trials. The Cas9 nuclease is instructed to make a site-
directed double-strand DNA break utilising the short
RNA molecule as a guide in the CRISPR/Cas9 system.
This technology has advanced significantly in recent
years, and it is now often used to edit the genome to add,
remove, or modify certain genes. Additionally,
CRISPR/Cas9-based gene drive may have the ability to
stop the spread of vector-borne illnesses. Although
comprehensive testing is necessary to support these
claims, the bacterial and archaeal adaptive immune
systems may offer a therapeutic solution to previously
incurable illnesses. using regard to disease modelling and
therapy, we discuss current advances made using
CRISPR/Cas9 in this review, as well as what future
considerations should be made when utilising this
technology.
Keywords :
CRISPR/Cas9, Genome editing, Cas proteins, Tumour infiltrating leukocytes (TILs), Nonhomologous end- joining (NHEJ), Homology-directed repair (HDR)
