The rapid development of next generation
sequencing (NGS) tool and technologies over the past
few years, valuable insights have been gained into the
complex and diverse biological systems with diverse
range from microbial communities to cancerous genome.
The NGS base technologies based of genomics,
epigenomics and transcriptomics, are preoperationally
concentrated on individual cells characterization. As an
example, ScRNA sequencing can sort out complex and
rare cellular populations, revealing the gene regulatory
inter and intra relationships, and trace the progression
of individual cell lineages during cell development.
Researchers have previously undiscovered molecular
details in single-nuclei and single-cell sequencing
experiments. Since recent years, analytical approaches
and sequencing methods have increased rapidly. In this
review, our prime focus will be on challenges related to
single cell isolation (SCI) and library preparation,
analysis of ScRNA seq data by computational pipelines.
These sequencing technologies will be greatly facilitating
molecular biological improvement and bioinformatics
due to the availability of bioinformatics tools
Keywords : Single-cell RNA sequencing, alternative splicing, Next generation sequencing