Biocontrol Potential of Lipopeptide Bio Surfactant Produced by Bacillus sp. from Mangrove Ecosystem at Vellar-Coleroon Estuarine Complex against Early Blight of Tomato


Authors : Prithiviraj A; Parthasarathi. R.

Volume/Issue : Volume 8 - 2023, Issue 6 - June

Google Scholar : https://bit.ly/3TmGbDi

Scribd : https://tinyurl.com/yc8r69hv

DOI : https://doi.org/10.5281/zenodo.8037888

Abstract : Biosurfactant producing microorganisms Bacillus sp, isolated from the Rhizosphere soil area Mangorve ecosystem at Vellar –Coleroon Estuarine complex, Killai backwater, Chidambaram, Tamilnadu, India. The biosurfactant producing ability was tested in crude oil as the hydrocarbon in a Minimal Salt Medium (MSM). The isolate found to produce Biosurfactant which is evident from the rapid screening test. The biochemical and Fourier transform infrared spectroscopy studies reveal the Biosurfactant is a lipopeptide. The biocontrol potential of the crude Biosurfactant tested against Early blight of tomato (Solanum lycopersicum L.) is caused by Alternaria solani (Ellis & Martin), which is one of the most devastating diseases in tomatoes. Currently, the only way to stop the illness from spreading is to apply fungicides. In vitro experiments revealed that at a concentration of 3.00 g l-1 , the biosurfactant generated by this bacterial strain inhibited the growth of A. solani by 72 per cent. In the pot culture, a concentration of 1.50 g l-1 crude biosurfactant was adequate to completely inhibit A. solani.

Keywords : Biosurfactant, Biocontrol, Bacillus sp, Lipopeptide, Alternaria solani (Ellis & Martin).

Biosurfactant producing microorganisms Bacillus sp, isolated from the Rhizosphere soil area Mangorve ecosystem at Vellar –Coleroon Estuarine complex, Killai backwater, Chidambaram, Tamilnadu, India. The biosurfactant producing ability was tested in crude oil as the hydrocarbon in a Minimal Salt Medium (MSM). The isolate found to produce Biosurfactant which is evident from the rapid screening test. The biochemical and Fourier transform infrared spectroscopy studies reveal the Biosurfactant is a lipopeptide. The biocontrol potential of the crude Biosurfactant tested against Early blight of tomato (Solanum lycopersicum L.) is caused by Alternaria solani (Ellis & Martin), which is one of the most devastating diseases in tomatoes. Currently, the only way to stop the illness from spreading is to apply fungicides. In vitro experiments revealed that at a concentration of 3.00 g l-1 , the biosurfactant generated by this bacterial strain inhibited the growth of A. solani by 72 per cent. In the pot culture, a concentration of 1.50 g l-1 crude biosurfactant was adequate to completely inhibit A. solani.

Keywords : Biosurfactant, Biocontrol, Bacillus sp, Lipopeptide, Alternaria solani (Ellis & Martin).

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