Authors :
CHBEL Faiza; EL ARJI Marzouk; HARIF Mhamed; YAHIA Hakima; RACHID Mohamed; MADANI Abdellah; AZEDDOUG Houssein; MOSSAFA Houssein; QUESSAR Asmaa
Volume/Issue :
Volume 8 - 2023, Issue 8 - August
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/2kvhdupj
DOI :
https://doi.org/10.5281/zenodo.8320996
Abstract :
Allogeneic hematopoietic stem cell
transplantation is a curative treatment for malignant
hematologic diseases, leukemias, congenital and acquired
non-malignant diseases of a patient (recipient) by
replacing recipients cells by new one from a healthy
donor. Monitoring of hematopoietic chimerism after
allogenic transplantation is a useful tool for determining
the engraftment of donor cells and predicting the risk of
relapse of the original disease. Nowadays, diverses
techniques are used and differ from laboratory to
laboratory, which make data exchange and comparaison
between them difficult. Amplification of short tandem
repeats (PCR-STR) constitutes the gold standard method
for chimerism quantification, although more sensitive
PCR techniques have recently developed. This study was
carried out on 30 allografted patients in whom the status
of chimerism was analysed by the use of STRs included in
Investigator Id-plex plus kit (Qiagen). This is conducted
by taking samples of whole blood from these patients at
different times in post-allograft from which we extract
DNA and amplify STR markers and genotyped by
capillary electrophoresis. The STR profiles have been
generated for each sample including donor and recipient
samples taken before the allogeneic transplant (J0).
Profiles comparison obtained from the post-allograft
samples with the genotypes of the donor and the pre-
allograft recipient, permits to determine the status of
chimerism in these different patients. This work was
preceded by analyzing a set of 219 individual from
Morocco with the objective to establish their usefulness
for human identification. Allelic frequencies for the 15
short tandem repeat (STRs) loci were calculated. Results
show that the 15 loci are highly polymorphic. The
combined power of exclusion for the fifteen loci is
0.99999968 and the combined discrimination indice was
0.999999999999999985. The combined matching
probability for these loci reaches 1.59 10-18 which make
these loci very useful for personal identification casework
purposes in Morocco.
Keywords :
Chimerism; STR; Morocco; Allogenic Transplanted Patients; Investigator IDPlex Plus; Informativity; Polymorphism, Forensic Parameters
Allogeneic hematopoietic stem cell
transplantation is a curative treatment for malignant
hematologic diseases, leukemias, congenital and acquired
non-malignant diseases of a patient (recipient) by
replacing recipients cells by new one from a healthy
donor. Monitoring of hematopoietic chimerism after
allogenic transplantation is a useful tool for determining
the engraftment of donor cells and predicting the risk of
relapse of the original disease. Nowadays, diverses
techniques are used and differ from laboratory to
laboratory, which make data exchange and comparaison
between them difficult. Amplification of short tandem
repeats (PCR-STR) constitutes the gold standard method
for chimerism quantification, although more sensitive
PCR techniques have recently developed. This study was
carried out on 30 allografted patients in whom the status
of chimerism was analysed by the use of STRs included in
Investigator Id-plex plus kit (Qiagen). This is conducted
by taking samples of whole blood from these patients at
different times in post-allograft from which we extract
DNA and amplify STR markers and genotyped by
capillary electrophoresis. The STR profiles have been
generated for each sample including donor and recipient
samples taken before the allogeneic transplant (J0).
Profiles comparison obtained from the post-allograft
samples with the genotypes of the donor and the pre-
allograft recipient, permits to determine the status of
chimerism in these different patients. This work was
preceded by analyzing a set of 219 individual from
Morocco with the objective to establish their usefulness
for human identification. Allelic frequencies for the 15
short tandem repeat (STRs) loci were calculated. Results
show that the 15 loci are highly polymorphic. The
combined power of exclusion for the fifteen loci is
0.99999968 and the combined discrimination indice was
0.999999999999999985. The combined matching
probability for these loci reaches 1.59 10-18 which make
these loci very useful for personal identification casework
purposes in Morocco.
Keywords :
Chimerism; STR; Morocco; Allogenic Transplanted Patients; Investigator IDPlex Plus; Informativity; Polymorphism, Forensic Parameters
