Assessment of Reproductive Function of Rabbit Bucks Administered Black Pepper (Piper nigrum) Extract as Feed Additive


Authors : Ere, R. E.; Ere-Richard, A. A.; Otto O. B.

Volume/Issue : Volume 11 - 2026, Issue 2 - February


Google Scholar : https://tinyurl.com/3j6dzehc

Scribd : https://tinyurl.com/4vsy6h4f

DOI : https://doi.org/10.38124/ijisrt/26feb429

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Abstract : This research examined the effect of ethanolic extract of black pepper (Piper nigrum) on the reproductive capabilities of rabbit bucks when used as a feed additive. The study utilized twenty (20) composite-breed of rabbit bucks aged 18-20 weeks in a completely randomized design, divided into five groups (A - E) of four bucks each, with four replicates per group. Group A (control) received standard pelleted feed, while group B – E were supplemented with 100, 150, 200 and 250 mg/kg black pepper extract respectively. Diets were provided for 90 days, within which semen quality (macro- and microscopic traits) and hormonal levels were assessed in the animals. Results from this study revealed that black pepper extract (BPE) at 100 – 200 mg/kg positively influenced both macroscopic and microscopic semen quality characteristics as well as hormonal profiles of rabbit bucks. However, Inclusion levels at 250mg/kg BPE adversely impacted semen quality characteristics and reduced circulating levels of reproductive hormones (FSH, LH and testosterone) due to its toxicity. The optimal inclusion rate for best reproductive outcomes was determined to be 200mg/kg and therefore considered the recommended dosage.

Keywords : Black Pepper, Semen Parameters, Hormonal Assay, Rabbit Bucks

References :

  1. Abdallah, E.M and Abdalla, W.E. (2018). Black pepper fruit (Piper nigrum L.) as antibacterial agent: A mini-review. Journal of bacteriology and mycology. 6(2):141-145
  2. Ajuogu, P.K and Ajayi, F.O (2010). Breeding responses of Rabbits to artificial insemination in the humid tropics. Animal Production Research Advances, 6(1), 41-42.
  3. Ajuogu, P.K., Herbert, U., Ibeh, P.C., Nodu, M.B., Ukpabio, U.H., Onyegbule, C.G. and Akintola, A.O. (2018). Semen quality characteristics and testosterone levels of rabbit bucks fed Costus afer leaf. African Journal of Biotechnology.17(2), 24-28.
  4. Akpa, G.N., Yahaya, H.K. and Martin, U.C. (2012). The Effects of Age, Breed, Sire, Body weight and Ejaculate characteristics of Rabbit Bucks. International Journal of Animal and Veterinary Advances. 4(3): 191-194
  5. Almbro, M., Damian, K., Dowling., Leigh, W. and Simmons (2011). Effect of vitamin E and beta-carotene on sperm competitiveness. Ecology letter. 14(9):891-895.
  6. Asuquo, O. R., Oko, O. O., Brownson, E.S., Umoetuk, G.B and Utin, I. S (2013). Effects of ethanolic leaf extract of Spondias mombin on the pituitary gonadal axis of female Wistar rats. Asian Pacific J. Reprod. 2(3):169-173
  7. Bearden, H. J., Fuquay, J. W. and Willard, S. T. (2004). Applied Animal Reproduction. 6th edition. Pearson Prentice Hall.  New Jersey.
  8. Benhong Z., Zhenpeng, Q., Gang, L., Chun, L and J, Z. (2012). Spermicidal and antigonococcal effect of tannins from pomegranate ring. Journal of medicinal plant research. 7(6): pp.1334-1339
  9. Brewer, M. S (2011). “Natural antioxidants: sources, compounds, mechanisms of action, and potential applications.” Comprehensive Reviews in Food Science and Food Safety 10(4): 221-247.
  10. Chanda, D., Shanker, K., Pal, A., Luqman, S., Bawankule, D.U., Mani, D and Darokar, M.P. (2009). Safety evaluation of Trikatu, a generic ayurvedic medicine in charles foster rats. J. Toxicol. Sci., 34:99-108
  11. Chauhan NS, Rao ChV, Dixit VK (2007) Effect of Curculigo orchioides rhizomes on sexual behavior of male rats. Fitoterapia 78:530-534.
  12. Chen, X., Fei, G., Jianpeng, L., Suhao, B., Yong, C., Dongli, L., Yong, L., Tongliang, H., Xiaofang, C., Qiqi, Z., Qingquan, L and Ren-shan, G. (2018). Diverged effects of piperine on testicular development: stimulating leydig cell development but inhibiting spermatogenesis in rats. Journal frontiers in pharmacology. 9:244.
  13. Chinta, G., Coumar, M.S., and Periyasamy, L. (2017). Reversible testicular toxicity of piperine on male albino rats. Pharmacogn. Mag. 13, S525-S532. Doi: 10.4103/pm.pm_405_16.
  14. Erdost H., Akkoc, CGO., Ozfiliz N., İlhan T., Tutuncu S (2009). The histological       investigation on the testes of mice after an application of capsaicin. Veterinarski arhiv.  79(5):509-516
  15. Ewuola, E. O, Osaiyuwa, O.H, Sokunbi, O.A, Hassa n, M.E, Olakanmi, I.G and Halogen, A.S (2011). Semen qualities of normal and frizzle feather strains of domestic Cocks treated with or without tocopherol. Journal of Trop. Anim. Prod. Invest. 13(1):30-35.
  16. Gayatri, N and Sahu, R.K. (2011). Phytochemical Evaluation and Antioxidant activity of Piper cubeba and Piper nigrum. Journal of Applied pharmaceutical science 1(8):153-157.
  17. GenStat (2009). GenStat getting started version (12th ed.). Lawes Agricultural Trust, Rothamsted Experimental station UK.
  18. Hassan, H.E., Elamin, K.M., Yausif, L. A., Musa, A.M and Elkhaimey, M.A. (2012). Evaluation of body weight and some morphometry traits at various ages in local rabbits of Sudan. Journal of Animal Science Advances 2(4): 407-415.
  19. Himabindu, D and Arunkumar, H. (2017). Effect of Black pepper (Piper nigrum L.) on the keeping quality of spiced cottage cheese. Journal of food and Dairy technology. 5(4): pp.1-36.
  20. Hirata, N., Tokunaga, M., Naruto, S, and Matsuda, H. (2007). Testosterone 5α-reductase inhibitory active constituents of Piper nigrum leaf. Biol. Pharm. Bull.,30(12):2402-2405.
  21. Igbokwe, A.A., Iyasere, O.S., Sobayo, R.A., Iyasere, S., Animashaun, R.I., Balogun, F.A., Aganran, Z.O., Fasola, M.O., Adedokun, A.D., Lakehinde, O.A, Lasisi, S.O., Suleiman, M.R., Iyiola, J.D., Daramola, J.O. (2018). Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative parameters of goat spermatozoa cryopreserved with tiger nut milk extender. Journal of Reproduction in domestic Animals. 2019:1-9.
  22. International Rabbit Reproduction Group (2005). Guidelines for the handling of rabbit bucks and semen. World Rabbit Sci. 13:147-164. Doi:http://dx.doi.org/10.4995/wrs.2005.527.
  23. Llyas, S. (2007). Azoospermia dam pemulihannya Melalui Regulasi Apoptosis Sel Spermatogenik Tikus (Rattus Sp.) pada penyunti kan kombinasi Tu and MPA [Disertasi], Universitas Indonesia, Jakarta, 2007.
  24. Meghwal, M and Goswami, T.K (2013). Chemical composition, Nutritional, Medicinal and functional properties of black pepper. Open Access Scientific Reports. 1(2):1-5
  25. Micallef, I.A., Hays, M.M., Latif, A., Alhasan, R., and Sufi, S.B (1995). Serum binding of steroid tracers and its possible effects on direct steroids immune assay. Anim. Clin. Biochem. 32:566-574.
  26. Nie, G.J and Wenzel, J.G.W (2001). Adaptation of hypo-osmotic swelling test to assess functional integrity of stallion spermatozoa plasma membranes. Theriogenology 55:1005-1018.
  27. Oyeyemi, M.O. and Okediran, B.S. (2007). Testicular parameters and sperm morphology of chinchilla rabbits fed with different planes of soy meal. Int. J. Morphol.,25 (1):139-144.
  28. Rajesh, J., Gopichand, C., Dineshbabu, J., Bavatharini, S and Latha, P. (2014). Effect of piperine on goat epididymal spermatozoa: an in vitro study. Asian Journal of pharmaceutical and clinical research. 7(5):57-61.
  29. Rodriguez-Martinez, H. (2003). Laboratory semen assessment and prediction of fertility: Still Utopia? Reproduction in Domestic Animals. 38:312-318.
  30. Salmin, A. (2000). Pengaruh Kadar Gliserol dalam Pengencer Susu Skim dan Lama Ekuilibrasi Terhadap Kualitas Sperma Domba Pasca Pembekuan. Magister Pertanian Thesis. Program Pascasarjana universitas Padjadjaran, Bandung.
  31. Sokunbi, O. A (2008). Growth and reproductive potential of pubertal boars fed dietary need (Azadirachta Indica A. Just) Kernel. Journal of Tropical Anim. Prod. Invest. 11(1):1-6.
  32. Sreedhar, B. N and Anantha, B.K. (2016). Phytochemical screening and evaluation of anti-fertility activity of Dactyloctenium aegyptium in male albino rats. Asian pacific journal of reproduction. 5(1): pp.51-57.
  33. Sutyarso, Muhartono, M. Kanedi (2016).  The Effect of Fruit Extracts of Black Pepper on the Fertility Potential of Male Albino Mice. American Journal of Medical and Biological Research 4(1):1-4.
  34. Tia, W.E. Hz., Ika, P.S and Dicky, M.R. (2018). The effect of ethanol extract of piper nigrum L. Fruit on reproductive system in Adult male Wistar Rats: A spermatogenic cells. Indonesian J. Pharm. 29(3):136-144.
  35. Vijayakumar, R.S. and Nalini, N. (2006). Piperine, an active principle from Piper nigrum, modulates hormonal and apolipoprotein profiles in hyperlipidemic rats. J. Basic Clin. Physiol. Pharmacol., 17:71-86.
  36. Williams, A.C. and Ford, W.C. (2001). The role of Glucose in Supporting Motility and Capacitation in Human Spermatozoa. Journal of Andrology. 22:680-695.
  37. World Health Organization. WHO Laboratory manual for the examination of the Human semen. Ed: 5th, World Health organization Geniva, 2010.

This research examined the effect of ethanolic extract of black pepper (Piper nigrum) on the reproductive capabilities of rabbit bucks when used as a feed additive. The study utilized twenty (20) composite-breed of rabbit bucks aged 18-20 weeks in a completely randomized design, divided into five groups (A - E) of four bucks each, with four replicates per group. Group A (control) received standard pelleted feed, while group B – E were supplemented with 100, 150, 200 and 250 mg/kg black pepper extract respectively. Diets were provided for 90 days, within which semen quality (macro- and microscopic traits) and hormonal levels were assessed in the animals. Results from this study revealed that black pepper extract (BPE) at 100 – 200 mg/kg positively influenced both macroscopic and microscopic semen quality characteristics as well as hormonal profiles of rabbit bucks. However, Inclusion levels at 250mg/kg BPE adversely impacted semen quality characteristics and reduced circulating levels of reproductive hormones (FSH, LH and testosterone) due to its toxicity. The optimal inclusion rate for best reproductive outcomes was determined to be 200mg/kg and therefore considered the recommended dosage.

Keywords : Black Pepper, Semen Parameters, Hormonal Assay, Rabbit Bucks

Paper Submission Last Date
28 - February - 2026

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