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Assessment of Antioxidant, Antihemolytic and Cytotoxic Effect of Tridax Procumbens Linn. Methanol Extracts in HaCaT - Human Keratinocytes Cell Line


Authors : Sunitha Kumari K.; John Vithya S.

Volume/Issue : Volume 11 - 2026, Issue 6 - June


Google Scholar : https://tinyurl.com/5y77zarj

Scribd : https://tinyurl.com/3a95j32m

DOI : https://doi.org/10.38124/ijisrt/26jun909

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : There was a mild reduction in clotting time, during in vitro condition. Cytotoxicity evaluation of T. procumbens, carried out using standard in vitro methods (HaCaT - Human keratinocytes cell line studies), indicated dose-dependent cytotoxic effects of the extracts (IC50 value of 142.275µg/mL) and cell viability (non-significant at 6.25 µg/mL, compared to standard ascorbic acid; p < 0.0001 for doses 12.5 to 100 µg/mL). The study reveals antihemolytic action of the methanol extract at low concentration and moderate cytotoxic activity at higher concentrations, suggesting safe and potential applications in anticancer research, although further detailed investigations are required.

Keywords : Tridax procumbens; Hair Growth; Anti Hemolytic Activity; in Vitro Cytotoxicity; HaCaT-Cell Line Studies

References :

  1. Mahima Anil Nikam, Pranali Rajendra Ner, Janhavi Anil Nikam, Vaibhav Ramdas Jadhav, Khushali Rajendra Pagar. Review on Tridax Procumbens. International Journal for Multidisciplinary Research (IJFMR), Volume 6, Issue 5, September-October 2024, E-ISSN: 2582-2160.
  2. Amutha, R., Sudha, A. and Pandiselvi, P., Tridax procumbens (Coat Button) -A Gift of Nature: An Overview. Pharmacological Benefits of Natural Products First Edition. Chapter – 12. 2019; 193 - 212.
  3. Himanshu C. Chaudhari, Kiran P. Patil, Research & Reviews in Pharmacy and Pharmaceutical Sciences A Review on Medicinal Importance of Tridax Procumbens Linn. JPPS,Volume 11, Issue 2|, 2022 e-ISSN:2320-1215.
  4. Ashok Kumar K., Umamaheswari M Sivashanmugam A. T., Subhadradevi V., Subhasini N. & Ravi T.K. Antioxidant activities of Erythrina strict Roxb. Using various in vitro and ex vivo models. Oriental pharmacy and Experimental medicine. 2008; 8,266-278.
  5. Yen C C and P D Duh, “Antioxidant properties of methanolic extract from peanut hulls". J AM. Oil chem. Sec., vol. 70;1993. P.P.383-386.q
  6. Chang S T, W u J.H, wang S.X, Kang P.L, Yang N S and Shuyur L F, Antioxidant activity of extract from Acacia confuses bark and heart wood, Journal of agricultural and food chemistry, vol.49; 2001, PP.3420- 3424.
  7. Yang ZG, Sun HX, Fang WH. Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. Vaccine. 2005; 23(44): 5196-5203.
  8. Júnior, E. C. F., Costa, B. P., Freire, J. C. P., de Sousa Melo, W. O., de Luna Freire Pessôa, H., de Castro Gomes, D. Q., ... and Pereira, J. V. Use of erythrocytes in cytotoxicity and toxicity assays of medicinal plant extracts: analysis of their application and bibliometric study. Boletín Latinoamericano y del Caribe de Plantas medicinales y aromáticas, 2019;18(4).
  9. Dhivyavarshni R, Sagadevan P, Jayaram Jayaraj K, Chandar Shekar B, Ranjth Kumar R, Rathish Kumar S. Studies on phytochemical screening, Antibacterial potential and Hemostatic activity of Tridax procumbens. Drug Discovery, 2018, 12, 1-6.
  10. Manjusha Borde, Ujjwala Kulkarni, Harish Lalan, Apurva Mishra, Rita Dhar and Yashavant Deshmukh. Effect of various extracts of leaves of Tridax procumbens on human blood clotting time: A comparative in vitro study. J. Nat. Prod. Plant Resour., 2014, 4 (6):9-14.

There was a mild reduction in clotting time, during in vitro condition. Cytotoxicity evaluation of T. procumbens, carried out using standard in vitro methods (HaCaT - Human keratinocytes cell line studies), indicated dose-dependent cytotoxic effects of the extracts (IC50 value of 142.275µg/mL) and cell viability (non-significant at 6.25 µg/mL, compared to standard ascorbic acid; p < 0.0001 for doses 12.5 to 100 µg/mL). The study reveals antihemolytic action of the methanol extract at low concentration and moderate cytotoxic activity at higher concentrations, suggesting safe and potential applications in anticancer research, although further detailed investigations are required.

Keywords : Tridax procumbens; Hair Growth; Anti Hemolytic Activity; in Vitro Cytotoxicity; HaCaT-Cell Line Studies

Paper Submission Last Date
30 - June - 2026

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