Authors : Blessy Susan Bangera; Dr. Prajna K S; Dr. Vidya Shenoy

Volume/Issue : Volume 9 - 2024, Issue 8 - August

Google Scholar : https://shorturl.at/M4L8d

Scribd : https://shorturl.at/KLt0o

DOI : https://doi.org/10.38124/ijisrt/IJISRT24AUG916

Abstract : The world of Material Science has constantly evolved with newer materials being introduced constantly. One such material is Graphene which possessed excellent electrical, mechanical, thermal, optical, and biological properties due to which it has been used extensively in the fields of Optoelectronics, Energy harvesting, Films and Coatings, Water Filtration, Structural Engineering applications, Thermal management Devices and Sensors. Graphene-based Nanoparticles have also shown promising results in biomedicine, Tissue engineering scaffolds, Biomarker detectors, Biosensors, and Drug Delivery systems. Furthermore, extensive investigation is being performed on graphene-based Nanomaterials for their use in Dentistry, as it is shown to have promising results when incorporated into various Dental Restorative and Prosthetic Materials. This narrative review aims to give an overview of the application of graphene derivatives in dentistry, particularly on their application in Prosthodontics and Dental Implantology based on available research data and clinical studies. Further research is imperative to fully explore the potential of graphene to ensure its safe usage in dental practice.

Keywords : Nano Particles, Graphene Oxide, Prosthodontics, Implant Dentistry.

References :

1. Lamster IB. The 2021 WHO Resolution on Oral Health. international dental journal. 2021 Aug;71(4):279.
2. Bei HP, Yang Y, Zhang Q, Tian Y, Luo X, Yang M, Zhao X. Graphene-based nanocomposites for neural tissue engineering. Molecules. 2019 Feb 13;24(4):658.
3. Dasari Shareena TP, McShan D, Dasmahapatra AK, et al. A review on graphene-based nanomaterials in biomedical applications and risks in environment and health. Nanomicro Lett 2018 ;10(3):1-34.
4. Yanwu Z, Shanthi M, Weiwei C, Xuesong L, Ji W, Jeffrey R. Graphene and Graphene Oxide: Synthesis, Properties and Applications.J Bio Med Sci.2010;22(35):3906-3924
5. Nileshkumar D, Ricardo B,lntekhab .Graphene: A Versatile Carbon-Based Material for Bone Tissue Engineering. Crit. Rev. Solid State Mater. Sci. 2010;35:52-71.
6. Dongn HS, S.J.Qi.Realising the potential of graphene-based materials for biosurfaces - A future perspective. Nat. Nanotechnol. 2011:6:543-546.
7. Lakshmi KA, llsha Rao G, Arthiseethalakshmi S, Mohamed MS. The revolutionary era of Graphene in Dentistiy-a review. RGUHS J Med Sci. 2016;6(4):139-45.
8. Madhav VNV, Joshi S, Kulkarni M. Graphene: a game changer in prosthodontics and implant dentistry. Int J Res Med Sci 2022;10:1565-7.
9. Bonilla-Represa V, Abalos-Labruzzi C, Herrera-Martinez M, Guerrero-Pérez MO. Nanomaterials in dentistry: state of the art and future challenges. Nanomaterials. 2020 Sep 7;10(9):1770.
10. Terrones, M.; Botello-Méndez, A.R.; Campos-Delgado, J.; López-Urías, F.; Vega-Cantu, Y.I.; 
Rodriguez-Macias, F.; Elías, A.L.; Muñoz-Sandoval, E.; Cano-Márquez, A.G.; Charlier, J.C. Graphene and graphite nanoribbons: Morphology, properties, synthesis, defects and applications. Nano Today 2010, 5, 351–372.
11. Ramanathan T, Abdala AA, Stankovich S, Dikin DA, et al. Functionalized graphene sheets for polymer nanocomposites. Nat. Nanotechnol. 2008; 3(6):327-31.
12. Yang J, Yan X, Wu M, et al. Self-assembly between graphene sheets and cationic poly (methyl methacrylate) (PMMA) particles: preparation and characterization of PMMA/graphene composites. J Nanopart Res 2012 ;14(1):1-9.
13. Heo S, Se Youn Cho, Do Hyeong Kim, Yun Sung Choi, Hyun Ho Park, Jin H. Improved Thermal Properties of Graphene Oxide-Incorporated Poly(methyl methacrylate) Microspheres. Journal of Nanoscience and Nanotechnology. 2012 Jul 1;12(7):5990–4.
14. Song J, Zhang J, Lin C. Influence of Graphene Oxide on the Tribological and Electrical Properties of PMMA Composites. Journal of Nanomaterials. 2013 Jan 1;2013:1–5.
15. Alamgir M, Nayak GC, Mallick A, et al. Processing of PMMA nanocomposites containing biocompatible GO and TiO2 nanoparticles. Mater. Manuf. Process 2018 ;33(12):1291-8.
16. Khan AA, Mirza EH, Mohamed BA, et al. Physical, mechanical, chemical and thermal properties of nanoscale graphene oxide-poly methylmethacrylate composites J Compos Mater 2018 ;52(20):2803- 13.
17. Lee JH, Jo JK, Kim DA, et al. Nano-graphene oxide incorporated into PMMA resin to prevent microbial adhesion. Dent Mater 2018 ;34(4):e63-72.
18. Reem Gamal, Gomaa YA, Ashraf Matrawy. Incorporating nano graphene oxide to poly-methyl methacrylate; antibacterial effect and thermal expansion. Journal of Modern Research (Online). 2019 Jul 1;1(1):19–2
19. Di Carlo S, De Angelis F, Brauner E, et al. Flexural strength and elastic modulus evaluation of structures made by conventional PMMA and PMMA reinforced with graphene. Eur Rev Med Pharmacol Sci. 2020; 24(10):5201-8
20. Bacali C, Baldea I, Moldovan M, Carpa R, Olteanu DE, Filip GA, et al. Flexural strength, biocompatibility, and antimicrobial activity of a polymethyl methacrylate denture resin enhanced with graphene and silver nanoparticles. Clinical Oral Investigations. 2019 Nov 16;24(8):2713–25.
21. Rayannavar S, Shaha BS, Shankargouda S, Nelogi S. Antifungal efficacy of tissue conditioner incorporated with nanographene oxide: An in-vitro study. IJDSIR. 2020;3:601-7.
22. Khan AA, De Vera MA, Mohamed BA, Javed R, Al‐Kheraif AA. Enhancing the physical properties of acrylic resilient denture liner using graphene oxide nanosheets. Journal of Vinyl and Additive Technology. 2022 Aug;28(3):487-93.
23. Kulshrestha S, Khan S, Meena R, Singh BR, Khan AU. A graphene/zinc oxide nanocomposite film protects dental implant surfaces against cariogenicStreptococcus mutans. Biofouling. 2014 Nov 26;30(10):1281–94.
24. Jung, H. S., Lee, T., Kwon, I. K., Kim, H. S., Hahn, S. K., and Lee, C. S. (2015). Surface Modification of Multipass Caliber-Rolled Ti alloy with Dexamethasone-Loaded Graphene for Dental applicationsResearch Support, Non-U.S. Gov't]. ACS Appl. Mater. Interfacesacs Appl. Mater. Inter. 7 (18), 9598–9607
25. Jin J, Zhang L, Shi M, Zhang Y, Wang Q. Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity. International Journal of Nanomedicine. 2017 Jun;Volume 12:4209–24.
26. Li H, Gao C, Tang L, Wang C, Chen Q, Zheng Q, et al. Lysozyme (Lys), Tannic Acid (TA), and Graphene Oxide (GO) Thin Coating for Antibacterial and Enhanced Osteogenesis. ACS applied bio materials. 2019 Dec 27;3(1):673–84.
27. Zhang C, Wang F, Jiang Z, Lan J, Zhao L, Si P. Effect of graphene oxide on the mechanical, tribological, and biological properties of sintered 3Y–ZrO2/GO composite ceramics for dental implants. Ceramics International. 2021 Mar 1;47(5):6940–6.
28. Jang W, Kim HS, Alam K, Ji MK, Cho HS, Lim HP. Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis. International Journal of Nanomedicine [Internet]. 2021 Aug 24 [cited 2023 Jul 3]; 16:5745–54.
29. Yong Cheol Shin, Jong Hyang Bae, Jong Ho Lee, Iruthayapandi Selestin Raja, Moon Sung Kang, Kim B, et al. Enhanced osseointegration of dental implants with reduced graphene oxide coating. Biomaterials Research. 2022 Mar 21;26(1).
30. Saloni P, Meshramkar R, Nadiger RK, Lekha K. A study to evaluate the antifungal efficacy of nanographene oxide incorporated in maxillofacial prosthesis material–An in vitro study. International Journal of Current Advanced Research, 10(06), pp. 24593-24596. DOI: http://dx.doi.org/10.24327/ijcar.2021.4898.24596
31. Li H, Xie Y, Li K, Huang L, Huang S, Zhao B, et al. Microstructure and wear behavior of graphene nanosheets-reinforced zirconia coating. Ceramics International. 2014 Sep;40(8):12821–9.
32. Su J, Chen Y, Huang Q. Graphene nanosheet-induced toughening of yttria-stabilized zirconia. Applied Physics A. 2016 Dec 17;123(1).
33. Zeng Z, Liu Y, Chen W, Li X, Zheng Q, Li K, et al. Fabrication and properties of in situ reduced graphene oxide‐toughened zirconia composite ceramics. Journal of the American Ceramic Society. 2018 Feb 20;101(8):3498–507.
34. Williams AG, Moore E, Thomas A, Johnson JA. Graphene-based materials in dental applications: antibacterial, biocompatible, and bone regenerative properties. International Journal of Biomaterials. 2023 Feb 7;2023.
35. C. Guo, R. Lu, X. Wang, and S. Chen, “Antibacterial activity, bio-compatibility and osteogenic diferentiation of graphene oxide coating on 3D-network poly-ether-ether-ketone for orthopaedic implants,” Journal of Materials Science: Mate- rials in Medicine, vol. 32, no. 11, 2021.
36. S. Z. Wu, Y. J. Liu, H. Zhang, and L. Lei, “Nano-graphene oxide improved the antibacterial property of antisense yycG RNA on Staphylococcus aureus,” Journal of Orthopaedic Surgery and Research, vol. 14, no. 1, 2019.
37. M. Pinto, I. C. Goncalves, and F. D. Magalhaes, “Graphene-based materials biocompatibility: a review,” Colloids and Surfaces B: Biointerfaces, vol. 111, pp. 188–202, 2013.
38. M. S. Kang, H. J. Jang, S. H. Lee et al., “Potential of carbon- based nanocomposites for dental tissue engineering and regeneration,” Materials, vol. 14, pp. 5104–5117, 2021.
39. C. Z. Liao, Y. C. Li, and S. C. Tjong, “Graphene nano- materials: synthesis, biocompatibility, and cytotoxicity,” International Journal of Molecular Sciences, vol. 19, no. 11, 2018.
40. Duch MC, Budinger GS, Liang YT, Soberanes S, Urich D, Chiarella SE, Campochiaro LA, Gonzalez A, Chandel NS, Hersam MC, Mutlu GM. Minimizing oxidation and stable nanoscale dispersion improves the biocompatibility of graphene in the lung. Nano letters. 2011 Dec 14;11(12):5201-7.
41. Akhavan O, Ghaderi E, Akhavan A. Size-dependent genotoxicity of graphene nanoplatelets in human stem cells. Biomaterials. 2012 Nov 1;33(32):8017-25.

42. Daulbayev C, Sultanov F, Korobeinyk AV, Yeleuov M, Taurbekov A, Bakbolat B, Umirzakov A, Baimenov A, Daulbayev O. Effect of graphene oxide/hydroxyapatite nanocomposite on osteogenic differentiation and antimicrobial activity. Surfaces and Interfaces. 2022 Feb 1;28:101683.
43. Ge Z, Yang L, Xiao F, Wu Y, Yu T, Chen J, Lin J, Zhang Y. Graphene family nanomaterials: properties and potential applications in dentistry. International journal of biomaterials. 2018 Dec 9;2018.
44. Dreanca A, Sarosi C, Parvu AE, Blidaru M, Enacrachi G, Purdoiu R, Nagy A, Sevastre B, Oros NA, Marcus I, Moldovan M. Systemic and local biocompatibility assessment of graphene composite dental materials in experimental mandibular bone defect. Materials. 2020 May 31;13(11):2511.
45. Moharamzadeh K, Brook IM, Van Noort R. Biocompatibility of resin-based dental materials. Materials. 2009 Apr 28;2(2):514-48.
46. BabsOn AL, Phillips GE. A rapid colorimetric assay for serum lactic dehyurogenase. Clinica Chimica Acta. 1965 Aug 1;12(2):210-5.
47. Wu S, Xu J, Zou L, Luo S, Yao R, Zheng B, Liang G, Wu D, Li Y. Long-lasting renewable antibacterial porous polymeric coatings enable titanium biomaterials to prevent and treat peri-implant infection. Nature Communications. 2021 Jun 3;12(1):3303.
48. Guazzo R, Gardin C, Bellin G, Sbricoli L, Ferroni L, Ludovichetti FS, Piattelli A, Antoniac I, Bressan E, Zavan B. Graphene-based nanomaterials for tissue engineering in the dental field. Nanomaterials. 2018 May 20;8(5):349.
49. Koc D, Dogan A, Bek B. Bite force and influential factors on bite force measurements: a literature review. European journal of dentistry. 2010 Apr;4(02):223-32.
50. Qin W, Wang C, Jiang C, Sun J, Yu C, Jiao T. Graphene oxide enables the    reosteogenesis of previously contaminated titanium in vitro. Journal of Dental Research.   2020 Jul;99(8):922-9.
51. Syama S, Mohanan PV. Safety and biocompatibility of graphene: A new generation nanomaterial for biomedical application. International journal of biological macromolecules. 2016 May 1;86:546-55.
52. Vera-Sánchez M, Aznar-Cervantes S, Jover E, Garcia-Bernal D, Onate-Sanchez RE, Hernández-Romero D, Moraleda JM, Collado-González M, Rodríguez-Lozano FJ, Cenis JL. Silk-fibroin and graphene oxide composites promote human periodontal ligament stem cell spontaneous differentiation into osteo/cementoblast-like cells. Stem cells and development. 2016 Nov 15;25(22):1742-54.
53. Liao KH, Lin YS, Macosko CW, Haynes CL. Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. ACS applied materials & interfaces. 2011 Jul 27;3(7):2607-15.
54. Akhavan O, Ghaderi E, Akhavan A. Size-dependent genotoxicity of graphene nanoplatelets in human stem cells. Biomaterials. 2012 Nov 1;33(32):8017-25.
55. Assali M, Almasri M, Kittana N, Alsouqi D. Covalent functionalization of graphene sheets with different moieties and their effects on biological activities. ACS Biomaterials Science & Engineering. 2019 Dec 10;6(1):112-21.
56. Mannoor MS, Tao H, Clayton JD, Sengupta A, Kaplan DL, Naik RR, Verma N, Omenetto FG, McAlpine MC. Graphene-based wireless bacteria detection on tooth enamel. Nature communications. 2012 Jan;3(1):763.
57. Zhao W, Xing Y, Lin Y, Gao Y, Wu M, Xu J. Monolayer graphene chemiresistive biosensor for rapid bacteria detection in a microchannel. Sensors and Actuators Reports. 2020 Nov 1;2(1):100004.
58. Pandit S, Li M, Chen Y, Rahimi S, Mokkapati V, Merlo A, Yurgens A, Mijakovic I. Graphene-based sensor for detection of bacterial pathogens. Sensors. 2021 Dec 3;21(23):8085.
59. Li B, Tan H, Anastasova S, Power M, Seichepine F, Yang GZ. A bio-inspired 3D micro-structure for graphene-based bacteria sensing. Biosensors and Bioelectronics. 2019 Jan 1; 123:77-84.
60. Priyadarsini S, Mohanty S, Mukherjee S, Basu S, Mishra M. Graphene and graphene oxide as nanomaterials for medicine and biology application. Journal of Nanostructure in Chemistry. 2018 Jun; 8:123-37.
61. Peña-Bahamonde J, Nguyen HN, Fanourakis SK, Rodrigues DF. Recent advances in graphene-based biosensor technology with applications in life sciences. Journal of nanobiotechnology. 2018 Sep 22;16(1):75.