Antimicrobial Property of Over-the-Counter Essential Oils and Ayurvedic Powders against Aeromonas hydrophila Isolated from Labeo rohito


Authors : Anamika Anandi; Utkarsh Kumar; Dr. Shail Pragya Singh

Volume/Issue : Volume 8 - 2023, Issue 7 - July

Google Scholar : https://bit.ly/3TmGbDi

Scribd : https://tinyurl.com/2cyakrew

DOI : https://doi.org/10.5281/zenodo.8210419

Abstract : This study aimed to investigate the antimicrobial activity of Syzygium aromaticum, Lavandula augustifolia, Zingiber officinale and Allium sativum essential oils and also powders of Emblica officinalis and Triphala (Emblica officinalis, Terminalia bellerica and Terminalia chebula) against Aeromonas spp isolates from Labeo rohita commonly known as Rohu. Essential oils were obtained from Khadi India manufacturers and powders from Khadi Natural manufacturers. The antimicrobial activity of the oils was assessed using the disc diffusion assay and the minimum inhibitory concentration was calculated using the simple broth dilution method. Syzygium aromaticum, Lavandula augustifolia, Zingiber officinale, Allium sativum, Emblica officinalis powder, and Triphala powder had MIC values of 500, 500, 350, 25 μg/ml, 5, and 10 mg/ml, respectively. All substances had antibacterial property against Aeromonas hydrophila. Syzygium aromaticum was found to be most susceptible and Triphala powder was least susceptible according to the Disc diffusion method. These products can be used as complementary therapeutic and prophylactic agents against this pathogen, with antimicrobial or immunostimulant properties. However, further research is needed to determine the safety and efficacy of these substances in vivo and their interactions with this bacterium.

Keywords : Phytotherapy; Disc Diffusion Method; Aeromonas Hydrophila; Motile Aeromonas Septicemia (MAS); Clove Oil, Ayurvedic Powder.

This study aimed to investigate the antimicrobial activity of Syzygium aromaticum, Lavandula augustifolia, Zingiber officinale and Allium sativum essential oils and also powders of Emblica officinalis and Triphala (Emblica officinalis, Terminalia bellerica and Terminalia chebula) against Aeromonas spp isolates from Labeo rohita commonly known as Rohu. Essential oils were obtained from Khadi India manufacturers and powders from Khadi Natural manufacturers. The antimicrobial activity of the oils was assessed using the disc diffusion assay and the minimum inhibitory concentration was calculated using the simple broth dilution method. Syzygium aromaticum, Lavandula augustifolia, Zingiber officinale, Allium sativum, Emblica officinalis powder, and Triphala powder had MIC values of 500, 500, 350, 25 μg/ml, 5, and 10 mg/ml, respectively. All substances had antibacterial property against Aeromonas hydrophila. Syzygium aromaticum was found to be most susceptible and Triphala powder was least susceptible according to the Disc diffusion method. These products can be used as complementary therapeutic and prophylactic agents against this pathogen, with antimicrobial or immunostimulant properties. However, further research is needed to determine the safety and efficacy of these substances in vivo and their interactions with this bacterium.

Keywords : Phytotherapy; Disc Diffusion Method; Aeromonas Hydrophila; Motile Aeromonas Septicemia (MAS); Clove Oil, Ayurvedic Powder.

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