A number of bacteria have now become antibiotic-resistant. This increases the importance of UTI drugs. We report, here, the activity of ethanol extract, of Quercus infectoria galls against UTI pathogens . antibacterial activity of ethanolic extract. Quercus infectoria from Sudan market was macerated with ethanol 85 %, fractionated by colum chromatography using benzene ethyl acetate, ethanol, and 70% ethanol. Phytochemical analysis of the extracts revealed the presence of antimicrobial active agents such as alkaloids, phenols ,glycosides, flavonoids resins, saponins, and tannins and then were subjected to chemical compound analysis Phytochemical screening, total phenolic content, total flavonoids content, and total tannins content were obtained. Eschericia coli shown resistance to Ciprofloxacin except Gentamicin and Proteus mirabilis shown resistance Trimethoprim while Staphylococcus aureus, Eschericia coli , Proteus mirabilis, Bacillus subtilis shown sensitivity to the crude extract and all the fractions except for A3 ,A4 ,A5 tubes of serial dilution, Eschericia coli, Staphylococcus aureus, Proteus mirabilis, Bacillus subtilis the results were exhibited a high activity (MIZD) against Proteus mirabilis (19 mm) and Bacillus subtilis (20 mm )have shown highly sensitivity the Quercus infectoria extract while e . Eschericia coli have shown moderate sensitivity ( 17 mm) Staphylococcus aureus (12mm) shown low.

Keywords : Quercus Infectoria, Urinary Tract Infections, Pathogenic Bacteria, Natural Agents, Traditional Medicine.