Authors :
Anupama Maurya, Urmila Nishad, Meraj Al, Manoj Kumar Yadav, Vijay Kumar Yadav
Volume/Issue :
Volume 5 - 2020, Issue 3 - March
Google Scholar :
https://goo.gl/DF9R4u
Scribd :
https://bit.ly/2w8WayE
Abstract :
A new, simple, precise and stabilityindicating UPLC (Ultra Performance Liquid
Chromatography) method was developed and validated
for the simultaneous determination of Diuretic Drug of
thiazide class of Hydrochlorothiazide in urine sample.
It involved 150 mm × 2.1 mm, Acclaim column.
The separation was achieved on simple gradient
method. The mobile phase was composed 0.2% TFA in
water and 0.2% TFA in acetonitrile. The flow rate of
the mobile phase was set at 0.5 ml/min-1
for gradient
elution and column temperature was maintained at
40˚C. The detector wavelength was 271 nm for
Hydrochlorothiazide. The retention times of
Hydrochlorothiazide are 2.9 and 3.4 minutes;
respectively. The total run time was 6.0 minutes within
which two compounds and was separated. The
described method was validated with respect to system
linearity, precision and accuracy. The precision of the
assay method was evaluated by carrying out six
independent assays Hydrochlorothiazide. The described
method was linear over the range, 0 .625 to 10 µg·mL-1
for Hydrochlorothiazide. The detection limit for HCTZ
0.4602580, LOQ for HCTZ 1.534194 The method was
validated in terms of linearity, accuracy and
reproducibility. Recovery was in the range of 93 –
108%. The method is fast and is suitable for analysis of
hydrochlorothiazide in urine samples.
A new, simple, precise and stabilityindicating UPLC (Ultra Performance Liquid
Chromatography) method was developed and validated
for the simultaneous determination of Diuretic Drug of
thiazide class of Hydrochlorothiazide in urine sample.
It involved 150 mm × 2.1 mm, Acclaim column.
The separation was achieved on simple gradient
method. The mobile phase was composed 0.2% TFA in
water and 0.2% TFA in acetonitrile. The flow rate of
the mobile phase was set at 0.5 ml/min-1
for gradient
elution and column temperature was maintained at
40˚C. The detector wavelength was 271 nm for
Hydrochlorothiazide. The retention times of
Hydrochlorothiazide are 2.9 and 3.4 minutes;
respectively. The total run time was 6.0 minutes within
which two compounds and was separated. The
described method was validated with respect to system
linearity, precision and accuracy. The precision of the
assay method was evaluated by carrying out six
independent assays Hydrochlorothiazide. The described
method was linear over the range, 0 .625 to 10 µg·mL-1
for Hydrochlorothiazide. The detection limit for HCTZ
0.4602580, LOQ for HCTZ 1.534194 The method was
validated in terms of linearity, accuracy and
reproducibility. Recovery was in the range of 93 –
108%. The method is fast and is suitable for analysis of
hydrochlorothiazide in urine samples.
