A concurrent and promising effects has been achieved by using cow urine as a efficient antibacterial activity against gram-negative and gram-positive human pathogen. The present study aims to evaluate anti-microbial activity photo activated cow urine against pathogenic bacterial strains. It is estimated by agar well diffusion method.It is evident from the data presented that the zone of inhibition of photo activated Gir cow urine and Khillari cow urine against bacteria Escherichi coli and staphulococcus aureus is significant.The present investigation clearly demonstrate that the average width of inhibition zone at 50 micrilitre dose was 1.6cm.Therefore this pathogen can be well managed by using cow urine.

Keywords : Photo activated Gir cow urine and khillari cow urine, E.coli, S. aureus, well diffusion method.