Allelic Polymorphism of Insulin Like Growth Factor 1 Gene and its Effect on Growth Performance of FUNAAB Alpha Chickens


Authors : Ojoawo H.T; Ogunpaimo O. J; Ilori B. M, Wheto, M; Adeleke, M. A.; Adebambo, A. O.; Adebambo, O. A.

Volume/Issue : Volume 5 - 2020, Issue 4 - April

Google Scholar : http://bitly.ws/9nMw

Scribd : https://bit.ly/2W4VqVi

Insulin like Growth Factor 1 gene is a biological and positional candidate gene known to play an important role in growth, proliferation and differentiation, body composition, metabolism, skeletal characteristics, growth of adipose tissue and fat deposition in chicken. The polymorphisms of Insulin like Growth Factor 1 gene were investigated in the FUNAAB Alpha and Marshall Broiler chicken populations. A total of 642 FUNAAB Alpha dual purpose, 67 Marshall and 118 FUNAAB Alpha Broiler chickens were used. Blood samples were collected through the wing vein of each chicken with the use of a 5ml disposable syringe. Genomic Deoxyribonucleic acid was extracted from the blood samples using ZymobeadTM Deoxyribonucleic acid extraction kit which was amplified using Polymerase Chain Reaction. The Polymerase Chain Reaction products were electrophoresed and amplicons obtained were digested with HinfI which was then electrophoresed on 1.5% agarose gel. Results obtained indicated that there was no observed polymorphism in the exons of the Insulin like Growth Factor 1 gene. However, the restriction analysis revealed two alleles; A and C in the promoter region.

Keywords : FUNAAB Alpha.

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