Authors :
Lidya Anin; Hebert Adrianto; Hanna Tabita Hasianna Silitonga; Setyarina Indrasari; Kartika Buana Sari
Volume/Issue :
Volume 8 - 2023, Issue 6 - June
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/mvzs6mc5
DOI :
https://doi.org/10.5281/zenodo.8162310
Abstract :
Dengue fever is a disease transmitted by Ae.
aegypti. Controlling mosquitoes can use temephos.
Temephos larvicides act primarily on the enzyme
acetylcholinesterase (AChE) by inhibiting this enzyme.
Temephos has drawbacks due to its high risk of
resistance, so larvicides from natural ingredients such as
pandan leaf extract (P. amaryllifolius) can be an
alternative. The mechanism of action of P. amaryllifolius
larvicide is still unclear. The purpose of thid study is to
ascertain how LC85 pandan leaf methanol extract affects
AChE levels in Ae. aegypti. This is a true-experiment
design with only a post-test control group research
design. Tests were carried out by treating mosquito
larvae with P. amaryllifolius LC85 extract, aquades, and
temephos for 24 hours and measuring AChE levels with
ELISA Reader. The results showed that the AChE
enzyme levels of P. amaryllifolius LC85 extract had an
average AChE enzyme level of 147.19 + 70.87 units/l. The
AChE enzyme levels of larvae exposed to P.
amaryllifolius LC85, aquades, and temephos had a
significant difference (p <0.05). P. amaryllifolius has
potential as a larvicidal, with a mechanism of action as a
neurotoxin.
Keywords :
AChE enzyme; Ae. aegypti; Larvae; Pandanus amaryllifolius.
Dengue fever is a disease transmitted by Ae.
aegypti. Controlling mosquitoes can use temephos.
Temephos larvicides act primarily on the enzyme
acetylcholinesterase (AChE) by inhibiting this enzyme.
Temephos has drawbacks due to its high risk of
resistance, so larvicides from natural ingredients such as
pandan leaf extract (P. amaryllifolius) can be an
alternative. The mechanism of action of P. amaryllifolius
larvicide is still unclear. The purpose of thid study is to
ascertain how LC85 pandan leaf methanol extract affects
AChE levels in Ae. aegypti. This is a true-experiment
design with only a post-test control group research
design. Tests were carried out by treating mosquito
larvae with P. amaryllifolius LC85 extract, aquades, and
temephos for 24 hours and measuring AChE levels with
ELISA Reader. The results showed that the AChE
enzyme levels of P. amaryllifolius LC85 extract had an
average AChE enzyme level of 147.19 + 70.87 units/l. The
AChE enzyme levels of larvae exposed to P.
amaryllifolius LC85, aquades, and temephos had a
significant difference (p <0.05). P. amaryllifolius has
potential as a larvicidal, with a mechanism of action as a
neurotoxin.
Keywords :
AChE enzyme; Ae. aegypti; Larvae; Pandanus amaryllifolius.
