The advancement and latest technologies used
for genome editing has opened the door of targeting and
altering the genomic sequences, depending upon the
bacterial nucleases, in almost all eukaryotic cells.
Genomic editing has helped the scientists in
understanding the contribution of genetics in studying
pathological process of many diseases by creating
accurate animal and cellular models. This technique has
shown extraordinary potential and provided latest
approaches from basic research to advance biomedical
and biotechnological research. The idea of gene editing
technology has reached the clinical practice phase after
the discovery of programable gene editors like Zinc
Finger Nucleases (ZFN), Transcription Activator Like
Effector Nucleases (TALENs) and Clustered Regularly
Interspaced Short Palindromic Repeat (CRISPR)–Casassociated nuclease. This review will discuss all the three
strategies (ZFN, TALENS, CRISPR/case system) used for
genome editing and their application against the diseases
as well as their mode of action and delivery methods.
Keywords : Genome editing; Zinc Finger Nucleases (ZFN); Transcription Activator Like Effector Nucleases (TALENs); styling; Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)–Cas9.