Mother is a person most honorable occupation and who need more skills . The breastfeeding is basic to health and development of children and also important for mothers. Just as there is a no person who can replace the mother’s love there is no substitute for mothers milk. Breastfeeding refers to the processes by which a woman expels milk from her breast. The breast milk can be stored and feed to her baby at a later period time.  Objectives  To assess the existing level of knowledge regarding expression and storage of breast milk among antenatal mothers attending antenatal clinic, At H.S.K. hospital and research Centre Bagalkot.  To determine the effectiveness of structured teaching programme on knowledge regarding expression and storage of breast milk.  To find out the association between post-test knowledge scores with their selected socio- demographic variables.  Hypothesis:  H1: There is a significant difference between pre-test and post-test knowledge scores, regarding expression and storage of breast milk among antenatal mothers, who are attending antenatal clinic at H.S.K. hospital Bagalkot.  H2: There is a significant association between post- test knowledge levels of antenatal mothers regarding expression and storage of breast milk with their selected socio-demographic variables.  Methods An evaluative approach with one group pre-test, post-test design was used. The sample consisted of 50 antenatal mothers attending antenatal clinic at H.S.K. hospital Bagalkot. They were chosen by convenient sampling technique of Non-probability type. The study was conducted at H.S.K Hospital and research center Bagalkot. The data was collected before and after administration of structured teaching programme. Structured knowledge questionnaire was used to collect data. A structured teaching programme was developed to assess the knowledge regarding expression and storage of breast milk among antenatal mothers attending antenatal clinic at H.S.K Hospital and research center Bagalkot.  Results The findings of the study concluded that antenatal mothers had inadequate level of knowledge regarding expression and storage of breast milk. The structured teaching programme was effective in improving the knowledge of the antenatal mothers.  Interpretation And Conclusion The findings of the study concluded that antenatal mothers had inadequate knowledge regarding expression and storage of breast milk. The STP was highly effective in improving the knowledge of antenatal mothers regarding expression and storage of breast milk.