A study to assess the effectiveness of self instructional module on knowledge regarding standard precaution on prevention of infection among staff nurses of selected hospital in Indore MP. The research design used for study was Pre-experimental one group pre-test post-test research design. A Quantitative evaluatory approach was used in this study. The tool for study was self-structured knowledge questionnaire which consists of 2 parts-PART- I consisted questions related to Sociodemographic data, PART-II consisted of self -structured awareness questionnaire to assess the effectiveness of self instructional module on knowledge regarding standard precaution on prevention of infection among staff nurses of selected hospital. The data was analyzed by using descriptive & inferential statistical methods. In pre-test knowledge score and it is reflected that exactly few ( 3.3%) staff nurses observed with poor level of knowledge (0-6) about standard precaution on prevention of infection, twenty four (40.0%) staff nurses showed average (7-12) level of knowledge, thirty four (56.7%) were in (13-18) level of knowledge, none (0.0%) of the staff nurses identified with excellent (19-24) level of knowledge about standard precaution on prevention of infection.