Background WHO recommended exclusive breast feeding upto 6 months of age till 2 years or beyond. This study attempts to compared the knowledge among primiparous and multiparous on Exclusive Breast Feeding residing at selelcted areas of kancheepuram district and the researchers proves the association between knowledge of the mothers and exclusive breast feeding.  Methods The mothers knowledge on exclusive breast feeding was determined by structured questionnaire. 100 mothers with infants of 0 to 6 months ( Primiparous 50 mothers and Multiparous 50 mothers)residing at selected areas of kancheepurm district.  Results Among 0 – 6 months old infants, the prevalence rate of exclusive breast feeding was about 45.5%, that is among multiparous it is about 49.3 and primiparous mothers it was about 39.4%. this study results states that the knowledge of the multiparous mothers was significantly high with the mean score of 8.49 than the primiparous mothers mean score is about 7.93.  Conclusions The study findings will be useful particularly forprimiparous mothers in order to gain knowledge on exclusive breast feeding practices and also it imparts awareness on exclusive breast feeding benefits for the mother as well as for the infant.