Authors : Aneena Wahab; Sreya Menon; Anjali Anne Jacob

Volume/Issue : Volume 10 - 2025, Issue 4 - April

Google Scholar : https://tinyurl.com/2ye5et6u

Scribd : https://tinyurl.com/2xpxwk9n

DOI : https://doi.org/10.38124/ijisrt/25apr2200

Google Scholar

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Note : Google Scholar may take 15 to 20 days to display the article.

Abstract : Blood is one of the most critical type of biological fluid recovered from crime scene and often crucial in reconstructing events, identifying individuals and time since deposition. This study focuses on the microbial aspect of the blood as blood provide an ideal environment for microbial growth due to its nutrient content. Microbial growth in dried bloodstains occurs in distinct phases and is influenced by environmental conditions such as temperature, humidity and exposure to air. The suggested approach is experimental, requires blood samples that are deposited on any surface and collected at various time intervals (6, 24, 48, 72, 96 hours). The study aims to investigate microbial growth dynamics in bloodstains over time and their impact on the spectral properties measured through UV-Vis spectrophotometer. The turbidometeric analysis measured the absorbance of rehydrated sample at 600nm to quantify microbial proliferation. This approach bridges forensic science and microbiology, ensuring a foundation for further researches in future.

Keywords : Bloodstains; Microbial Growth; Incubate; Centrifuge; Absorbance; Time Intervals.

References :

1. Quraishi R, Lakshmy R, Mukhopadhyay AK, Jailkhani BL. Analysis of the stability of urea in dried blood spots collected and stored on filter paper. Ann Lab Med. 2013 May;33(3):190-2. doi: 10.3343/alm.2013.33.3.190. Epub 2013 Apr 17. PMID: 23667845; PMCID: PMC3646193.
2. Robinson JM, Pasternak Z, Mason CE, Elhaik E. Forensic Applications of Microbiomics: A Review. Front Microbiol. 2021 Jan 13;11:608101. doi: 10.3389/fmicb.2020.608101. PMID: 33519756; PMCID: PMC7838326.
3. Nodari R, Arghittu M, Bailo P, Cattaneo C, Creti R, D’Aleo F, Saegeman V, Franceschetti L, Novati S, Fernández-Rodríguez A, Verzeletti A. Forensic Microbiology: When, Where and How. Microorganisms. 2024 May 14;12(5):988.
4. Dash HR, Das S. Microbial degradation of forensic samples of biological origin: potential threat to human DNA typing. Molecular biotechnology. 2018 Feb;60(2):141-53.
5. Gouello A, Dunyach-Remy C, Siatka C, Lavigne JP. Analysis of Microbial Communities: An Emerging Tool in Forensic Sciences. Diagnostics (Basel). 2021 Dec 21;12(1):1. doi: 10.3390/diagnostics12010001. PMID: 35054168; PMCID: PMC8774847.
6. Speruda M, Piecuch A, Borzęcka J, Kadej M, Ogórek R. Microbial traces and their role in forensic science. J Appl Microbiol. 2022 Apr;132(4):2547-2557. doi: 10.1111/jam.15426. Epub 2022 Jan 5. PMID: 34954826.
7. Huntress A. OD600 Measurements: An Easy Guide for Researchers Using Bacterial Cultures: bitesizebio.